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Vitamin B12 is a limiting factor for in vivo reprogramming

ABSTRACT: Vitamin B12 is a limiting factor for in vivo reprogramming

PROVIDER: PRJNA645207 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12188077_1.fastq.gz	Fastqsanger.gz
	SRR12188077_2.fastq.gz	Fastqsanger.gz
	SRR12188078_1.fastq.gz	Fastqsanger.gz
	SRR12188078_2.fastq.gz	Fastqsanger.gz
	SRR12188079_1.fastq.gz	Fastqsanger.gz

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Vitamin B12 is a limiting factor for in vivo reprogramming

Project description:Reprogramming in vivo using OCT4, SOX2, KLF4 and MYC (OSKM) triggers cell dedifferentiation, which is considered of relevance for tissue repair and regeneration. However, little is known about the metabolic requirements of this process. We found that antibiotic depletion of the gut microbiota abolished in vivo reprogramming. Analysis of bacterial metagenomes from stool samples of wild type (WT) and OSKM mice treated with doxycycline led us to identify vitamin B12 as a key factor for in vivo reprogramming, which is partly supplied by the microbiome. We report that B12 demand increases during reprogramming due to enhanced expression of enzymes in the methionine cycle, and supplementing B12 levels both in vitro and in vivo enhances the efficiency of OSKM reprogramming.

2023-08-07 | GSE154149 | GEO

RNASeq samples of OSKM reprogrammable MEFs treated with or without vitamin B12

Project description:Examination of the impact of vitamin B12 supplementation during in vitro OSKM reprogramming

2023-08-07 | GSE200579 | GEO

Transthyretin orchestrates vitamin B12-induced stress resilience

Project description:Background: Chronic stress significantly contributes to mood- and anxiety disorders. Previous data suggest a correlative connection between vitamin B12 supplementation, depression, and stress resilience. However, the underlying mechanisms are still poorly understood. Methods: Using the chronic variable stress mouse model coupled with RNA-sequencing, we determined vitamin B12-induced transcriptional changes related to stress resilience. By viral-mediated gene transfer and in vivo epigenome editing, we reveal a functional pathway linking vitamin B12, DNA methylation, and depressive-like symptoms. Results: We identified Transthyretin (Ttr) as a sex-specific key target of vitamin B12 action in chronic stress. Accordingly, TTR expression was increased postmortem in the prefrontal cortex of male, but not female, depressed patients. Virally altered Ttr in the prefrontal cortex functionally contributed to stress- and depression-related behaviors, changes in dendritic spine morphology, and gene expression. In stressed mice, vitamin B12 reduced DNAme in the Ttr promoter region. Importantly, using in vivo epigenome editing to alter DNAme in the brains of living mice for the first time, we establish a direct causal link between DNAme on Ttr and stress-associated behaviors. Discussion: In summary, using state-of-the-art techniques, this study uncovers a mechanistic link between cobalamin supplementation, Ttr, and markers of chronic stress and depression, encouraging further studies into dietary interventions for mood disorders.

2024-05-13 | GSE267047 | GEO

Transthyretin orchestrates vitamin B12-induced stress resilience [II]

2024-05-18 | GSE267339 | GEO

ChIPSeq of H3k36me3 samples of OSKM reprogramming mouse embryonic fibroblasts treated with B12

Project description:Examination of the impact of vitamin B12 supplementation during in vitro OSKM reprogramming on H3K36me3

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New Roles for Vitamin B12 Regulation

Project description:Elucidation of new roles for vitamin B12 in regulation of folate, ubiquinone, and methionine metabolism

2018-10-22 | PXD005723 | Pride

RNA-seq for understanding effects of vitamin B12 removal on astrocyte culture

Project description:Astrocytes express a vitamin B12 uptake receptor, CD320/TCblR, that is regulated by S1P1 signaling. In search of genes controlled under B12 deficiency in astrocytes, we employed RNA-seq using RNA of astrocytes cultured in normal or B12 deficient media.

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Mutations of the selected strain growing in low levels of Vitamin B12 in Mycobacterium marinum

Project description:Vitamin B12 (B12) is an important cofactor in mycobacterial metabolism, and some pathogenic mycobacteria need to obtain it from the host. In this study, we investigated the transport of vitamin B12 in Mycobacterium marinum. We identified a transcriptor regulator that could be potentially involved in the uptake process. RNA sequencing analysis were performed in order to elucidate the regulon of this new transcriptor.

2023-08-14 | GSE240448 | GEO

Effects of vitamin B12 on the gene expression of PAO1 under anaerobic growth conditions

Project description:Pseudomonas aeruginosa undergoes cell elongation and forms robust biofilms during anaerobic respiratory growth using nitrate (NO3-) as an alternative electron acceptor. Understanding the mechanism of cell shape change induced upon anaerobiosis is crucial to the development of effective treatments against P. aeruginosa biofilm infection. Anaerobic growth of PAO1 reached higher cell density in the presence of vitamin B12, an essential coenzyme of class II ribonucleotide reductase. In addition, cell morphology returned to a normal rod shape. These results suggest that vitamin B12, the production of which was suppressed during anaerobic growth, can restore cellular machineries for DNA replication and therefore facilitate better anaerobic growth of P. aeruginosa with normal cell division. We used microarray to elucidate the global gene expression profiles underlying vitamin B12-induced changes in bacterial cell shape and growth-associated properties. Gene expression profiles of PAO1 grown in LBN (LB+NO3-) or LBN supplemented with 1 microM vitamin B12 are compared.

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Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

Project description:Recent functional genomics and genome-scale modeling approaches indicated that B12 production in Lactobacillus reuteri could be improved by medium optimization. Here we show that a series of systematic single amino acid omissions could significantly modulate the production of B12 from nearly undetectable levels (by isoleucine omission) to 20-fold higher than previously reported through omission of cysteine. We analyzed by cDNA microarray experiments the transcriptional response of L. reuteri to the medium lacking cysteine. These results supported the observed high B12 production and provided new avenues for future improvement of production of vitamin B12. Keywords: cell type comparison loop design

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