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A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion [RNA-Seq]

ABSTRACT: A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion [RNA-Seq]

PROVIDER: PRJNA645384 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12193457.fastq.gz	Fastqsanger.gz
	SRR12193458.fastq.gz	Fastqsanger.gz
	SRR12193459.fastq.gz	Fastqsanger.gz
	SRR12193460.fastq.gz	Fastqsanger.gz
	SRR12193461.fastq.gz	Fastqsanger.gz

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A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion [ChIP-Seq]

Project description:To explore what FOXL2-dependent changes of gene expression observed in our RNA-seq data were directly related to FOXL2 binding, we performed ChIP-seq analysis in FOXL2-expressing granulosa cells (two data points). To explore the potential co-regulation between FOXL2 and ESR2 of several targets, we knocked-down Esr2 in granulosa cells and performed a ChIP-seq under the same conditions as those previously described for FOXL2.

2021-03-24 | GSE154581 | GEO

A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion [RNA-Seq]

Project description:We depleted Foxl2 or Esr2 in murine primary granulosa cells and assessed the molecular consequences at the transcrit levels. We show that FOXL2 is involved in a large number of regulatory actions essential for the maintenance of cell fate of granulosa cells. We confirm that FOXL2 and ESR2 modulate, directly of through a coherent feed-forward loop, an important number of common targets.

2021-03-24 | GSE154185 | GEO

A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion

Project description:A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion

| PRJNA646724 | ENA

A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion [ChIP-Seq]

Project description:A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion [ChIP-Seq]

| PRJNA646726 | ENA

FOXL2 binding sites in primary cells from mouse ovarian follicles

Project description:FOXL2 is a lineage determining transcription factor in the ovary, but its direct targets and modes of action are not fully characterized. Here, we explore the genomic targets of FOXL2. We found in particular that FOXL2 directly modulates Esr2 expression through a newly identified intronic element. Input DNA and FOXL2 ChIP

2014-10-29 | E-GEOD-60858 | biostudies-arrayexpress

A genome-wide exploration of the targets of the transcription factors FOXL2 and ESR2 unveils their implication in cell migration, invasion and adhesion

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-24 | GSE154582 | GEO

FOXL2 binding sites in primary cells from mouse ovarian follicles

2014-10-29 | GSE60858 | GEO

FOXL2 interaction with different binding partners regulates the dynamics of granulosa cell differentiation across ovarian development

Project description:FOXL2 is a transcription factor essential for female fertility, expressed in somatic cells of the ovary, notably granulosa cells. In the mouse, Foxl2 deletion leads to partial sex reversal postnatally. However, deletion of the gene in 8-week-old females leads to granulosa to Sertoli cell transdifferentiation. We hypothesised that different outcomes of Foxl2 deletion in embryonic versus adult ovary may depend on a different role played across ovarian development. Therefore, we characterised the dynamics of gene expression and chromatin accessibility changes in purified murine granulosa cells across key developmental stages (E14.5, 1 and 8 weeks). We then performed genome-wide identification of FOXL2 target genes and on-chromatin interacting partners by ChIP-SICAP. We found that FOXL2 regulates more genes at postnatal stages, through the interaction with factors regulating primordial follicle activation (PFA), such as NR5A2, and others regulating steroidogenesis including AR and ESR2. As a proof of principle experiment, we chose one FOXL2 interactor, Ubiquitin specific protease 7 (USP7) and showed that deletion of this gene in granulosa cells leads to a blockage of PFA, impaired ovary development and sterility. Our study constitutes a comprehensive resource for exploration of the molecular mechanisms of ovarian development and causes of female infertility.

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Transcription profiling of mouse KK1 granulosa cells transfected with Foxl2-VP16 and Foxl2-MAD

Project description:The Foxl2 transcription factor is required for ovarian function during follicular development. Our approach to begin to understand Foxl2 function is through the identification of Foxl2 regulated genes in the ovary. Transiently transfected KK1 mouse granulosa cells were used to identify genes that are potentially regulated by Foxl2. KK1 cells were transfected in three groups (mock, activated, and repressed) and twenty-four hours later RNA was isolated and submitted for Affymetrix microarray analysis. Experiment Overall Design: To increase the potential of Foxl2 to alter gene expression levels of putative target genes, two fusions were constructed consisting of Foxl2 fused to the activation domain of the Herpes simplex virus VP16 transcription factor (Foxl2-VP16) and Foxl2 fused to the repression domain of the murine MAD transcription factor (Foxl2-MAD). Levels of gene expression were compared between mock transfected cells and those that were transfected with Foxl2-VP16 and Foxl2-MAD, respectively.

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Genome-wide maps of ESR2 chromatin state in Mantle cell lymphoma cells

Project description:In order to elucidate chromatin binding regions of ESR2 in Mantle cell lymphoma, we used ESR2 antibodies, opti-mized chromatin-immunoprecipitation (ChIP), and performed ChIP-Seq of ESR2 over-expressed Granta-519 cells and Granta519-mock cells.We present for the first time, the cistrome of nuclear receptor ESR2 in Mantle cell lymphoma cell line.

2022-07-01 | GSE190599 | GEO

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