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Microarray analysis of long noncoding RNA expression profile in pORF5-transfected HeLa cells

ABSTRACT: Microarray analysis of long noncoding RNA expression profile in pORF5-transfected HeLa cells

PROVIDER: PRJNA645918 | ENA |

REPOSITORIES: ENA

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Microarray analysis of long noncoding RNA expression profile in pORF5-transfected HeLa cells

Project description:Numerous studies have provided evidence for the involvement of lncRNAs in bacterial infections, including Mycobacterium tuberculosis, Helicobacter pylori, Listeria monocytogenes, and so forth. However, few studies have evaluated the alternation of lncRNAs in obligate intracellular bacterium C. trachomatis.The molecular mechanisms underlying pathogenesis of C. trachomatis are still elusive, and lncRNAs may provide new insights into the potential mechanisms. pORF5 (Accession number: NP_040384) is the only secreted protein in eight plasmid-encoded proteins in C. trachomatis. Our previous studies also verified that pORF5 influenced the expression of host proteins.Here, we performed a microarray analysis to detect the global lncRNAs and mRNAs expression in pORF5-transfected HeLa cells, and tried to identify pORF5-related lncRNAs. Our study may help to understand the interplay between lncRNAs and coding genes anticipated in the pathogenesis of C. trachomatis.

2020-07-14 | GSE154341 | GEO

Microarray analysis of long noncoding RNA expression profile in HeLa cells infected Chlamydia trachomatis

Project description:Microarray analysis of long noncoding RNA expression profile in HeLa cells infected Chlamydia trachomatis

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Assessment Of Long Noncoding RNA CCAT1 In Colorectal Cancer Patients

Project description:1. Evaluate the diagnostic value of long noncoding RNA (CCAT1) expression by RT-PCR in peripheral blood in colorectal cancer patients versus normal healthy control personal. 2. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in diagnosis of colorectal cancer patients & its relation to tumor staging. 3. Evaluate the clinical utility of detecting long noncoding RNA (CCAT1) expression in precancerous colorectal diseases. 4. Compare long noncoding RNA (CCAT1) expression with traditional marker; carcinoembryonic antigen (CEA) and Carbohydrate antigen 19-9 (CA19-9) in diagnosis of colorectal cancer.

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HeLa CD4+ cells transfected with parental vector pCep4

Project description:Expression profiles of HeLa CD4+ cells transfected with parental vector pCep4. Cells are treated with nocodazole and hydroxyurea. Keywords: time-course

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RIP: miR-202 mimic-transfected HeLa cells vs. negative control

Project description:Potential miR-202 targets were identified using a targetome-wide RIP-based microarray. HeLa cells were transfected with either a miR-202 mimic or a scrambled single-stranded RNA negative control. RNA was isolated from total cell lysate prior to IP and from antibody-immobilized Protein G agarose beads-RNP complexes (post-IP).

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Microarray analysis of long noncoding RNA expression profile in HeLa cells infected Chlamydia trachomatis

Project description:Chlamydia trachomatis (C. trachomatis) is an intracellular bacterium, and is one of the main pathogens that cause sexually transmitted infections worldwide. Long non-coding RNAs (lncRNAs) have become vital regulators in many biological processes. However, few studies have shown that lncRNAs take part in the pathogenesis of C. trachomatis. Here, we used microarrays to study the expression profiles of lncRNAs and mRNAs in HeLa cells at 12, 24, and 40 hours pot-infection (hpi). Our study provides evidence that lncRNAs are involved in the interaction between C. trachomatis and hosts.

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HeLa CD4+ cells transfected with epitope-tagged eTat plasmids

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cDNA microarray analysis of miR-1 transfected HeLa and NPC-TW01 cells

Project description:To investigate the mechanisms of miR-1 inducing apoptosis, we performed cDNA expression microarray analysis to identify the candidate miR-1 regulating genes that are involved in apoptosis. NPC-TW01 cells were transfected with miR-1 or miR-negative control for 40 hours, their total RNA were isolated from the cells and hybridized to an Agilent human whole genome oligo 4 x 44 K microarray. HeLa cells were transfected with miR-1 or miR-negative control for 40 hours, their total RNA were isolated from the cells and hybridized to an Agilent human whole genome oligo 4 x 44 K microarray.

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Expression data from HeLa cells transfected with plasmid expressing Salmonella effector SrfJ or HeLa cells transfected with the empty vector

Project description:SrfJ is an effector of the type III secretion systems of the Gram-negative intracellular pathogen Salmonella enterica serovar Typhimurium. To study the effects of this effector on global gene expression in host cells, we have expressed SrfJ on human HeLa cells through transient transfection. The comparison with HeLa cells transfected with a plasmid not expressing SrfJ, revealed a number of genes that are differentially expressed when SrfJ is present.

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