Project description:Lines nearly isogenic for fw3.2 in the cultivated background Solanum lycopersicum c.v. Yellow Stuffer were grown in the greenhouse in a completely randomized design. fw3.2 (ys) and fw3.2 (wt) are NILs carrying cultivated and wild alleles for fw3.2 locus. Young flower buds were harvested. For each sample, three replicates were used. RNA was extracted using Trizol and Stand-specific libraries were prepared from total RNA and sequences of 51 bp were generated on an Illumina HiSeq2000.