Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Inovirus M13 strain:NEB PhD-12 kit

ABSTRACT: High-throughput phage display screening of S100 protein family

PROVIDER: PRJNA646756 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12244812.fastq.gz	Fastqsanger.gz
	SRR12244813.fastq.gz	Fastqsanger.gz
	SRR12244628.fastq.gz	Fastqsanger.gz
	SRR12244629.fastq.gz	Fastqsanger.gz
	SRR12244636.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Inovirus M13

Project description:M13 bacteriophage random peptide phage display libraries selected for binding to serum antibodies

| PRJNA196935 | ENA

Inovirus M13

Project description:Inovirus M13 Targeted Locus (Loci)

| PRJNA270317 | ENA

Inovirus M13

Project description:Inovirus M13 Raw sequence reads

| PRJNA397152 | ENA

Inovirus M13

Project description:Bacteriophage M13 Raw sequence reads

| PRJNA356430 | ENA

Inovirus M13

Project description:Profiling active site specificity for proteases ADAMTS13 and Thrombin

| PRJNA356764 | ENA

Inovirus M13

Project description:Inovirus M13 Raw sequence reads

| PRJNA316731 | ENA

Inovirus M13 strain:SP063

Project description:Development of potent in vivo mutagenesis plasmids with broad mutational spectra

| PRJNA294841 | ENA

Inovirus M13 strain:SP055-rpoZ-cMyc-Cry1Ac1(d123)-kanR

Project description:Phage-assisted continuous evolution of Cry1Ac to bindTrichoplusia ni CAD3

| PRJNA293870 | ENA

Antigen Specific VNAR Screening in Whitespotted Bamboo Shark (Chiloscyllium plagiosum) with Next Generation Sequencing

Project description:IgNAR exhibits significant promise in the fields of cancer and anti-virus biotherapies. Notably, the variable regions of IgNAR (VNAR) possess comparable antigen binding affinity with much smaller molecular weight (~12 kDa) compared to IgNAR. Antigen specific VNAR screening is a changeling work, which limits its application in medicine and therapy fields. Though phage display is a powerful tool for VNAR screening, it has a lot of drawbacks, such as small library coverage, low expression levels, unstable target protein, complicating and time-consuming procedures. Here we report VNAR screening with next generation sequencing (NGS) could effectively overcome the limitations of phage display, and we successfully identified approximately 3000 BAFF-specific VNARs in Chiloscyllium plagiosum vaccinated with the BAFF antigen. The results of modelling and molecular dynamics simulation and ELISA assay demonstrated that one out of the top five abundant specific VNARs exhibited higher binding affinity to the BAFF antigen than those obtained through phage display screening. Our data indicates NGS would be an alternative way for VNAR screening with plenty of advantages.

2024-01-01 | GSE249557 | GEO

Project description:SCOPE system-based peptide screening against EphA2 by phage display

| PRJNA882610 | ENA