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Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-Biotinylation

ABSTRACT: Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-Biotinylation

PROVIDER: PRJNA646757 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11119477.fastq.gz	Fastqsanger.gz
	SRR11119478.fastq.gz	Fastqsanger.gz
	SRR11119479.fastq.gz	Fastqsanger.gz
	SRR11119480.fastq.gz	Fastqsanger.gz
	SRR11119481.fastq.gz	Fastqsanger.gz

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Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-Biotinylation

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Project description:Re-activation and clonal expansion of tumor-specific antigen (TSA)-reactive T cells are critical to the success of checkpoint blockade and adoptive transfer of tumor-infiltrating lymphocyte (TIL)-based therapies. There are no reliable markers to specifically identify the repertoire of TSA-reactive T cells due to their heterogeneous composition. We introduce FucoID as a general platform to detect endogenous antigen-specific T cells for studying their biology. Through this interaction-dependent labeling approach, intratumoral TSA-reactive CD4+, CD8+ T cells, and TSA-suppressive CD4+ T cells can be detected and separated from bystander T cells based on their cell-surface enzymatic fucosyl-biotinylation. Compared to bystander TILs, TSA-reactive TILs possess a distinct T cell receptor (TCR) repertoire and unique gene features. Although exhibiting a dysfunctional phenotype, TSA-reactive CD8+ TILs possess substantial capabilities of proliferation and tumor-specific killing. Featuring genetic manipulation-free procedures and a quick turnover cycle, FucoID should have the potential of accelerating the pace of personalized cancer treatment.

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