Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: HIV-1, BIV, EIAV, FIV, and MLV integration into human cells raw sequence reads

PROVIDER: PRJNA647337 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12321000_1.fastq.gz	Fastqsanger.gz
	SRR12321000_2.fastq.gz	Fastqsanger.gz
	SRR12321001_1.fastq.gz	Fastqsanger.gz
	SRR12321001_2.fastq.gz	Fastqsanger.gz
	SRR12321002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 139

Similar Datasets

Homo sapiens

Project description:MLV integration junction sequence in human CD34+ cells

| PRJNA236553 | ENA

Homo sapiens strain:K562 cells

Project description:MLV integration sites in human K562 cells

| PRJNA188712 | ENA

Homo sapiens

Project description:SIN-MLV retroviral integration sites in human HPCs

| PRJNA179168 | ENA

Homo sapiens

Project description:Integration sites of HIV in Homo sapiens HEK293 cells raw sequence reads

| PRJNA295930 | ENA

Homo sapiens

Project description:Homo sapiens Raw sequence reads for HIV-1 integration sites

| PRJNA1045670 | ENA

Homo sapiens

Project description:Homo sapiens Raw sequence reads for HIV-1 integration sites

| PRJNA552059 | ENA

Homo sapiens foetal mesenchymal stem cell miRNA transcriptome

Project description:We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We report that the majority of miRNA genes encode mature isomers that vary in size by one or more bases at the 3’ and/or 5’ end of the miRNA. Northern blotting for individual miRNAs showed that the proportions of isomiRs expressed by a single miRNA gene often differ between cell and tissue types. IsomiRs were readily co-immunoprecipitated with Argonaute proteins in vivo and were active in luciferase assays, indicating that they are functional. Bioinformatics analysis predicts substantial differences in targeting between miRNAs with minor 5’ differences and in support of this we report that a 5’ isomiR-9-1 gained the ability to inhibit the expression of DNMT3B and NCAM2 but lost the ability to inhibit CDH1 in vitro. This result was confirmed by the use of isomiR-specific sponges. Our analysis of the miRGator database indicates that a small percentage of human miRNA genes express isomiRs as the dominant transcript in certain cell types and analysis of miRBase shows that 5’ isomiRs have replaced canonical miRNAs many times during evolution. This strongly indicates that isomiRs are of functional importance and have contributed to the evolution of miRNA genes Sequence library of miRNAs from a single sample of human foetal mesenchymal stem cells. Results tested and confirmed by northern blotting. Please note that only raw data files are available for the embryonic and neual samples and thus, directly submitted to SRA (SRX547311, SRX548700, respectively under SRP042115/PRJNA247767)

2014-08-06 | E-GEOD-58734 | biostudies-arrayexpress

Homo sapiens

Project description:HIV integration site in human genome

| PRJNA241020 | ENA

Homo sapiens immortalized MSCs: 3A6

Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.

2010-09-25 | GSE24338 | GEO