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Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells [PRO-seq]

ABSTRACT: Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells [PRO-seq]

PROVIDER: PRJNA647374 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12267707_1.fastq.gz	Fastqsanger.gz
	SRR12267707_2.fastq.gz	Fastqsanger.gz
	SRR12267708_1.fastq.gz	Fastqsanger.gz
	SRR12267708_2.fastq.gz	Fastqsanger.gz
	SRR12267709_1.fastq.gz	Fastqsanger.gz

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Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells [PRO-seq]

Project description:Precision run-on sequencing (PRO-seq) of nascent RNA synthesis across the human genome. The PRO-seq data was generated from human K562 erythroleukemia cells that were either unconditioned (unCond) or preconditioned with a single heat shock exposure (preC). After two day recovery from the preconditioning, during which the cells underwent two mitotic divisions, the cells were additionally subjected to a single heat shock to measure nascent transcription in response to heat shock in unconditioned versus preconditioned cells.

2021-04-01 | GSE154746 | GEO

Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells.

Project description:Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells.

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Heat-induced transcriptional memory profiled in human K562 erythroleukemia cells.

Project description:Precision run-on sequencing (PRO-seq) of nascent RNA synthesis across the human genome. The PRO-seq data was generated from human K562 erythroleukemia cells that were either unconditioned (unCond) or preconditioned with multiple heat shock exposures (preCond). After two day recovery from the preconditioning, during which the cells underwent two mitotic divisions, the cells were additionally subjected to a single heat shock to measure nascent transcription in response to heat shock in unconditioned versus preconditioned cells.

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Chromatin accessibility in human K562 erythroleukemia cells profiled upon heat shock, recovery, and heat-induced transcriptional memory

Project description:ATAC-seq in human K562 erythroleukemia cells that were either unconditioned (s_uC and HSS_uC), preconditioned with a single heat shock exposure (s_pC), or preconditioned with multiple heat exposures (HSS_pC). After two day recovery from the preconditioning, during which the cells underwent two mitotic divisions, the cells were additionally subjected to a single heat shock to analyze heat-induced changes in chromatin accessibility in unconditioned versus preconditioned cells.

2021-04-01 | GSE154744 | GEO

Chromatin accessibility in human K562 erythroleukemia cells profiled upon heat shock, recovery, and heat-induced transcriptional memory

Project description:Chromatin accessibility in human K562 erythroleukemia cells profiled upon heat shock, recovery, and heat-induced transcriptional memory

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K562 PRO-seq

Project description:K562 PRO-seq

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PRO-IP-seq: Pol II CTD code at nt-resolution in human K562 cells

Project description:Active sites of transcription were labelled with biotinylated nucleotide during a run-on reacation, where after an immunoprecipitation with antibodies that target Pol II CTD was conducted. This PRO-IP-seq protocol was conducted in human K562 erythroleukemia cells. The K562 cells were either untreated (NHS) or treated with 30 minutes of heat shock at 42°C (HS).

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Genome-wide binding sites of NF90/ILF3 in K562 erythroleukemia cells

Project description:We used ChIP-seq to characterize the genome-wide binding sites of NF90/ILF3 in K562 erythroleukemia cells.

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Project description:Analysis of differential gene expression during TPA-induced megakaryocyte differentiation of human erythroleukemia K562 cells. The transcription profile of untreated vs TPA-treated K562 cells for 2 days was compared using gene microarrays.

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Project description:Genome-wide binding sites of NF90/ILF3 in K562 erythroleukemia cells

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