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CHD7 regulates osteogenic differentiation of human dental follicle cells via PTH1R signaling

ABSTRACT: CHD7 regulates osteogenic differentiation of human dental follicle cells via PTH1R signaling

PROVIDER: PRJNA647804 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12279277_1.fastq.gz	Fastqsanger.gz
	SRR12279277_2.fastq.gz	Fastqsanger.gz
	SRR12279278_1.fastq.gz	Fastqsanger.gz
	SRR12279278_2.fastq.gz	Fastqsanger.gz
	SRR12279279_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

CHD7 regulates osteogenic differentiation of human dental follicle cells via PTH1R signaling

Project description:Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent chromatin remodeling enzyme, functioning as chromatin reader to conduct epigenetic modification. Its effect on osteogenic differentiation of human dental follicle cells (hDFCs) remains unclear. Here we show CHD7 expression increases with osteogenic differentiation. knockdown of CHD7 impairs the osteogenic ability of hDFCs, characterized by reduced alkaline phosphatase activity and mineralization, and decreased expression of osteogenesis-related genes. Conversely, CHD7 overexpression enhances the osteogenic differentiation of hDFCs. Mechanically, RNA-seq analyses revealed the down-regulated enrichment of PTH (parathyroid hormone)/PTH1R (parathyroid hormone receptor-1) signaling pathway after CHD7 knockdown. We found the expression of PTH1R positively correlates with CHD7. Importantly, overexpression of PTH1R in CHD7-knockdown hDFCs partially rescued the impaired osteogenic differentiation. Our research demonstrates that CHD7 regulates the osteogenic differentiation of hDFCs by regulating the transcription of PTH1R.

2020-07-22 | GSE154822 | GEO

Expression data from human dental follicle cells

Project description:We analysed the genexpression of dental follicle cells (DFCs) after 3 days osteogenic differentiation with BMP2 after transfection with a DLX3 plasmid (pDLX3) and after transfection with an empty plasmid (pEV) Total RNAs were isolated from dental follicle cells after transfection with pDLX3 and pEV and osteogenic induction with BMP2 at day 3

2014-08-01 | E-GEOD-59949 | biostudies-arrayexpress

Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro

Project description:We analysed gene expression profiles in dental follicle cells before and after osteogenic differentiation with dexamethasone.

2009-09-12 | GSE18072 | GEO

CHD7 Regulates Osteogenic Differentiation of Human Dental Follicle Cells via PTH1R Signaling

Project description:Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent chromatin remodeling enzyme, functioning as chromatin reader to conduct epigenetic modification. Its effect on osteogenic differentiation of human dental follicle cells (hDFCs) remains unclear. Here, we show the CHD7 expression increases with osteogenic differentiation. The knockdown of CHD7 impairs the osteogenic ability of hDFCs, characterized by reduced alkaline phosphatase activity and mineralization, and the decreased expression of osteogenesis-related genes. Conversely, the CHD7 overexpression enhances the osteogenic differentiation of hDFCs. Mechanically, RNA-seq analyses revealed the downregulated enrichment of PTH (parathyroid hormone)/PTH1R (parathyroid hormone receptor-1) signaling pathway after CHD7 knockdown. We found the expression of PTH1R positively correlates with CHD7. Importantly, the overexpression of PTH1R in CHD7-knockdown hDFCs partially rescued the impaired osteogenic differentiation. Our research demonstrates that CHD7 regulates the osteogenic differentiation of hDFCs by regulating the transcription of PTH1R.

| S-EPMC7525296 | biostudies-literature

Expression data from human dental follicle cells

2014-08-01 | GSE59949 | GEO

Gene expression profiles of dental follicle cells after 7 days of differentiation in vitro with BMP2, IGF2 and dexamethasone

Project description:We analysed gene expression profiles in dental follicle cells after 7 days of osteogenic differentiation with different inducers. Total RNAs were isolated from dental follicle cells after 7 days of differentiation with dexamethasone, BMP2, IGF2 and for control with standard cell culture medium

2010-11-10 | E-GEOD-20963 | biostudies-arrayexpress

Gene expression profiles of dental follicle cells after 7 days of differentiation in vitro with BMP2, IGF2 and dexamethasone

Project description:We analysed gene expression profiles in dental follicle cells after 7 days of osteogenic differentiation with different inducers.

2010-03-20 | GSE20963 | GEO

PTHrP+ dental follicle cells

Project description:PTHrP+ dental follicle cells

| PRJNA994951 | ENA

Comparative gene expression analysis of dental follicle and periodontal ligament in humans

Project description:The aim of this study was to evaluate and compare the gene expression profiles of dental follicle and periodontal ligament in humans, which can possibly explain their functions of dental follicle and PDL such as eruption coordination and stress resorption. That may apply this information to clinical problem like eruption disturbance and to periodontal tissue engineering. PDL samples were obtained from permanent premolars (n=11) and dental follicle samples were obtained during extraction of supernumerary teeth (n=4). Comparative cDNA microarray analysis revealed several differences in gene expression between permanent PDL and dental follicles.

2014-08-21 | E-GEOD-51342 | biostudies-arrayexpress

Gene expression profiles of dental follicle cells after seven days of ostoeogenic differentiation

Project description:We analysed gene expression profiles in dental follicle cells after seven days of ostoeogenic differentiation Total RNAs were isolated from dental stem cells at day 0 (before differentiation) and after 7 days of differentiation)

2013-07-19 | E-GEOD-49007 | biostudies-arrayexpress

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