Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis

ABSTRACT: Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis

PROVIDER: PRJNA648378 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12318105.fastq.gz	Fastqsanger.gz
	SRR12318106.fastq.gz	Fastqsanger.gz
	SRR12318107.fastq.gz	Fastqsanger.gz
	SRR12318108.fastq.gz	Fastqsanger.gz
	SRR12318109.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 86

Similar Datasets

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis

Project description:In the Caenorhabditis elegans germline, thousands of mRNAs are concomitantly expressed with antisense 22G-RNAs, which are loaded into the Argonaute CSR-1. Despite their essential functions for animal fertility and embryonic development, how CSR-1 22G-RNAs are produced remains unknown. Here, we show that CSR-1 slicer activity is primarily involved in triggering the synthesis of small RNAs on the coding sequences of germline mRNAs and post-transcriptionally regulates a fraction of targets. CSR-1-cleaved mRNAs prime the RNA-dependent RNA polymerase, EGO-1, to synthesize 22G-RNAs in phase with ribosome translation in the cytoplasm, in contrast to other 22G-RNAs mostly synthesized in germ granules. Moreover, codon optimality and efficient translation antagonize CSR-1 slicing and 22G-RNAs biogenesis. We propose that codon usage differences encoded into mRNA sequences might be a conserved strategy in eukaryotes to regulate small RNA biogenesis and Argonaute targeting

2021-04-23 | PXD020293 | Pride

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [Ribo-Seq]

Project description:Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [Ribo-Seq]

| PRJNA648380 | ENA

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [RIP-Seq]

Project description:Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [RIP-Seq]

| PRJNA648382 | ENA

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [GRO-Seq]

Project description:Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [GRO-Seq]

| PRJNA648379 | ENA

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [ncRNA-seq]

Project description:Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [ncRNA-seq]

| PRJNA648383 | ENA

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [RNA-Seq]

Project description:Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [RNA-Seq]

| PRJNA648381 | ENA

Translation and codon usage regulate Argonaute slicer activity to trigger germline small RNA biogenesis [ncRNA-seq]

Project description:In Caenorhabditis elegans germline, thousands of mRNAs are concomitantly expressed with antisense 22G-RNAs, which are loaded into the Argonaute CSR-1. Despite their essential functions for animal fertility and embryonic development, how CSR-1 22G-RNAs are produced remain unknown. Here, we show that CSR-1 slicer activity is primarily involved in triggering the synthesis of small RNAs on the coding sequences of germline mRNAs and post-transcriptionally regulates a fraction of targets. CSR-1-cleaved mRNAs prime the RNA-dependent RNA polymerase, EGO-1 to synthesize 22G-RNAs concurrently with ribosome translation in the cytoplasm, in contrast to other 22G-RNAs mostly synthesized in germ-granules. Moreover, differences in germline mRNA codon optimality and ribosome occupancy antagonistically affect the abundance of CSR-1 22G-RNAs and the stability of their mRNA targets. We propose that codon usage differences encoded into mRNA sequences might be a conserved strategy adopted in eukaryotes to regulate small RNA biogenesis and Argonaute targeting.

2021-04-22 | GSE155076 | GEO