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ABSTRACT: Transcriptome of Rose flower

PROVIDER: PRJNA648687 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR12329579_1.fastq.gz	Fastqsanger.gz
	SRR12329579_2.fastq.gz	Fastqsanger.gz
	SRR12329580_1.fastq.gz	Fastqsanger.gz
	SRR12329580_2.fastq.gz	Fastqsanger.gz
	SRR12329581_1.fastq.gz	Fastqsanger.gz

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affy_duplicature_lyon_rose. Double flower phenomenon: Control of petal number in roses

Project description:affy_duplicature_lyon_rose. The objective is to identify the genes involved in petal doubling in rose. In this study we are using two rosa gallica genotypes: wild-type (simple flower rose) and Cardinal de Richelieu (double flower rose), and two rosa hybrida genotypes : Souvenir de la Malmaison, which has about 110 petal, and its bud sport cultivar, Souvenir de St Anne’s. In this study, we used a microarray approach to compare the transcriptome of double flower rose (CDR) versus simple flower rose (G). The objective is to identify genes whose expression is associated with the double flower phenotype. These genes are putative candidates involved in the control of petal organ number per flower. Floral buds were dissected under a microscope and pooled in eppendorf tubes. Tissue samples were harvested at the same time during 3 weeks in April 2007. Total RNA was extracted from the pools of flowers using the Plant RNA kit (Macherey Nagel), and then used to hybridize Rosa-Affymetrix microarrays. Keywords: genotype comparison

2011-09-01 | GSE18353 | GEO

affy_petaldvt_lyon_rose: Flower/Petal initiation and development in roses

Project description:affy_petaldvt_lyon_rose. The objective is to identify genes involved in petal development in rose. We aim at identifying genes whose expression correlates with flower opening and scent emission. In this study, we used a microarray approach to compare the transcriptome of a scented rose flower (PF) versus non-scented rose flower (RF). Samples (petal tissues) were collected at the same time early in the afternoon. Total RNA was extracted using the Plant RNA kit (Macherey Nagel), and then used to hybridize Rosa-Affymetrix microarrays. Keywords: scented vs non-scented flowers

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affy_duplicature_lyon_rose. Double flower phenomenon: Control of petal number in roses

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affy_petaldvt_lyon_rose: Flower/Petal initiation and development in roses

2011-08-31 | E-GEOD-18357 | biostudies-arrayexpress

Project description:Transcriptome of Rose flower

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An RNA-Seq atlas of gene expression in rose flower under treatment of various phytohormones and plant growth regulators

Project description:Using RNA-seq, we recently investigated the transcriptomic dynamics of rose flower under treatment of various plant hormones, including ethylene, 2,4-D, NAA, cytok, gibberellins, abscisic acid, brassinosteroids, salicylic acid, jasmonates, as well as ethylene inhibitor 1-MCP and AgNO. We obtained approximately 240GB data and dissected the transcriptional network with the aim of exploring the transcriptional variation of rose responses towards those plant hormones. Our data will be useful to all those working with the analysis of rose gene expression.

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Expression profiles of water deficit stress (WDS) regulated genes in rose petals

Project description:Water deficit stress (WDS) is a crucial factor that causes the inhibition of petal expansion and abnormal flower opening in rose. The regulatory mechanisms of petal expansion by WDS at transcriptional level were investigated by analysis expression profiles under WDS. Analysis used total RNA samples of petals taken from flowers treated by WDS comparison to those from control flowers. Transcriptome dynamics during treatment time responsive to WDS.

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affy_cinetique_lyon_rose: Flower/Petal initiation and development in roses

Project description:affy_cinetique_lyon_rose. The objective is to identify genes involved in petal development and senescence. R. chinensis cv Old Blush (OB) was used for the following reasons: it is a diploid Chinese rose that participated in the generation of modern roses (recurrent flowering, scent, etc.). The objective here is to identify genes whose expression is associated with different flower development stages, from floral meristem to senescing flower. These genes are putative candidates involved in floral initiation, development and senescence. All samples were collected at the same time early in the afternoon. Meristems and early flower development stages were dissected under a microscope. Total RNA was extracted from harvested tissues using the Plant RNA kit (Macherey Nagel), and then used to hybridize Rosa-Affymetrix microarrays. Keywords: time course 12 arrays - rose 6 developmental stages, 2 replicates each.

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Project description:early flower development of rose

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Expression profiles of water deficit stress (WDS) regulated genes in rose petals

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