Apis mellifera
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA648853 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12333210.fastq.gz | Fastqsanger.gz | |||
| SRR12333211.fastq.gz | Fastqsanger.gz | |||
| SRR12333212.fastq.gz | Fastqsanger.gz | |||
| SRR12333213.fastq.gz | Fastqsanger.gz | |||
| SRR12333214.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 12 |
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Project description:Female honeybees are specified as workers or queens based on diet during early development. Workers are essentially sterile with a reduced number of ovarioles and no spermatheca. In the presence of the queen (queen mandibular pheromone) and her brood, worker ovaries are kept in an inactive quiescent state. If the queen is removed, or lost, worker bees are able to sense this change in their environment and their ovaries undergo complete remodelling producing unfertilised haploid eggs that will produce male (drone bees). In this study we analyse gene expression in queen, worker, and laying worker ovaries using RNA-seq and explore differences in the chromatin landscape (focussing on H3K27me3).
2019-09-17 | GSE120560 | GEO
Project description:Female honeybees are specified as workers or queens based on diet during early development. Workers are essentially sterile with a reduced number of ovarioles and no spermatheca. In the presence of the queen (queen mandibular pheromone) and her brood, worker ovaries are kept in an inactive quiescent state. If the queen is removed, or lost, worker bees are able to sense this change in their environment and their ovaries undergo complete remodeling producing unfertilized haploid eggs that will produce male (drone bees). In this study we analyze gene expression in queen, worker, and laying worker ovaries using RNA-seq and explore differences in the chromatin landscape (focusing on H3K27me3).
2019-09-17 | GSE120561 | GEO
Project description:Transcriptional profiling of brain tissue of was performed by RNA-SEQ in females and males. The sex specific expressed and spliced genes were examined comparing drone and worker honeybees. Examination of mRNA profiles in heads of male and female honeybees pupae were compared.
2013-03-27 | E-GEOD-45408 | biostudies-arrayexpress
Project description:Transcriptional profiling of brain tissue of was performed by RNA-SEQ in workers, queens and males. The sex and caste specific expressed and spliced genes were examined comparing drone, worker and queen honeybees.
2013-03-27 | GSE45408 | GEO
Project description:Bisulfite sequence of brains of worker honeybees Apis mellifera
| PRJDB2080 | ENA
Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. Among the specialized structures in workers the hind tibia forms the corbicula or the pollen basket, a smooth region surrounded by a row of a long scopal hairs, used for carrying pollen and other materials to the nest. This morphological trait and the respective behaviour are absent in queens. Herein we show details of the initial steps of hind legs morphogenesis in honeybee castes. Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from hind leg imaginal discs of pre-pupa honeybees of both castes we present a list of differentially expressed genes.
2012-02-15 | GSE34293 | GEO
Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. Among the specialized structures in workers the hind tibia forms the corbicula or the pollen basket, a smooth region surrounded by a row of a long scopal hairs, used for carrying pollen and other materials to the nest. This morphological trait and the respective behaviour are absent in queens. Herein we show details of the initial steps of hind legs morphogenesis in honeybee castes. Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from hind leg imaginal discs of pre-pupa honeybees of both castes we present a list of differentially expressed genes. Analysis used one dye-swap combination to compare workers and queens hind leg development at prepupal stages when juvenile hormone titers is much higher in queens
2012-02-15 | E-GEOD-34293 | biostudies-arrayexpress
Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. The differential feeding regime experienced by the queen and the worker larvae of the honeybee Apis mellifera shapes a complex endocrine response cascade that ultimately sets up differences in brain morphologies. Herein we report on aspects of brain morphogenesis during larval development and the brain gene expression signature of fourth instar larvae (L4) of both castes, a developmental stage characterized by the greatest differences in juvenile hormone (JH) titers between castes Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from brain of fourth instar larvae honeybees of both castes we present a list of differentially expressed genes.
2012-12-31 | GSE39239 | GEO
Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. The differential feeding regime experienced by the queen and the worker larvae of the honeybee Apis mellifera shapes a complex endocrine response cascade that ultimately sets up differences in brain morphologies. Herein we report on aspects of brain morphogenesis during larval development and the brain gene expression signature of fourth instar larvae (L4) of both castes, a developmental stage characterized by the greatest differences in juvenile hormone (JH) titers between castes Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from brain of fourth instar larvae honeybees of both castes we present a list of differentially expressed genes. Analysis used one dye-swap combination to compare workers and queens brain development at fourth instar larvae when juvenile hormone titers is higher in queens.
2012-12-31 | E-GEOD-39239 | biostudies-arrayexpress
Project description:Epigenetic modifications are known to profoundly affect the development and behavior of social insects. In the well-known caste differentiation process of honeybee (Apis mellifera), female larvae with identical genomes are fed royal jellydifferently and develop into either normal workers or into very large, long-lived, and extremely fecund queens, and the queen-worker asymmetry of honeybee is known to be result largely to differential genomic imprinting during larval development that involves DNA methylation-based regulation. The discovery of reversible N6-methyladenosine (m6A) RNA methylation modification has defined a new era for RNA-metabolism-related genetic regulation, yet much remains unknown about m6A-mediated post-transcriptional regulatory mechanisms. Here, we report the first honeybee RNA m6A methylome. Specifically, we used the m6A-seq technique to examine the RNA m6A methylomes of honeybee larvae, including queen and worker larvae at multiple instar stages. We identified multiple conserved features of m6A methylation machinery and transcriptome-wide m6A distribution trends among insect species, and observed that m6A marks exert functions in regulating caste differentiation, with apparently particularly strong functional impacts on fifth instar worker larvae. Functional annotation of differentially methylated candidate caste-differentiation-related transcripts revealed many known regulators of caste differentiation (e.g. ILP-2, p110, PI3K, and JHAMT etc.) as well as the widely-studied Vitellogenin gene, which has not previously been implicated in caste differentiation. As ever-more regulatory roles for m6A marks are discovered, honeybees may become an excellent model studying the biology of such epi-transcriptomic regulatory systems, from embryonic development through holometabolous caste-specific development and on towards behavior and the emergent social hierarchies underlying eusociality in animals.
2020-12-13 | GSE137000 | GEO