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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle

ABSTRACT: The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle

PROVIDER: PRJNA648869 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12329800_1.fastq.gz	Fastqsanger.gz
	SRR12329800_2.fastq.gz	Fastqsanger.gz
	SRR12329801_1.fastq.gz	Fastqsanger.gz
	SRR12329801_2.fastq.gz	Fastqsanger.gz
	SRR12329802_1.fastq.gz	Fastqsanger.gz

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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle [PChi-C]

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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle [RNA-Seq]

Project description:Circadian gene expression is essential for organisms to adjust cellular responses and anticipate daily changes in the environment. In addition to its physiological importance, the clock circuit represents an ideal, temporally resolved, system to study transcription regulation. Here, we analysed changes in spatial mouse liver chromatin conformation using genome-wide and promoter-capture Hi-C alongside daily oscillations in gene transcription in mouse liver. We found circadian topologically associated domains switched assignments to the transcriptionally active, open chromatin compartment and the inactive compartment at different hours of the day while their boundaries stably maintain their structure over time. Individual circadian gene promoters displayed maximal chromatin contacts at times of peak transcriptional output and the expression of circadian genes and contacted transcribed regulatory elements, or other circadian genes, was phase-coherent. Anchor sites of promoter chromatin loops were enriched in binding sites for liver nuclear receptors and transcription factors, some exclusively present in either rhythmic or stable contacts. The circadian 3D chromatin maps provided here identify the scales of chromatin conformation that parallel oscillatory gene expression and protein factors specifically associated with circadian or stable chromatin configurations.

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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle [Hi-C]

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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle [PChi-C]

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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle [ChIP-seq]

2021-05-04 | GSE155149 | GEO

The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle.

Project description:BackgroundCircadian gene expression is essential for organisms to adjust their physiology and anticipate daily changes in the environment. The molecular mechanisms controlling circadian gene transcription are still under investigation. In particular, how chromatin conformation at different genomic scales and regulatory elements impact rhythmic gene expression has been poorly characterized.ResultsHere we measure changes in the spatial chromatin conformation in mouse liver using genome-wide and promoter-capture Hi-C alongside daily oscillations in gene transcription. We find topologically associating domains harboring circadian genes that switch assignments between the transcriptionally active and inactive compartment at different hours of the day, while their boundaries stably maintain their structure over time. To study chromatin contacts of promoters at high resolution over time, we apply promoter capture Hi-C. We find circadian gene promoters displayed a maximal number of chromatin contacts at the time of their peak transcriptional output. Furthermore, circadian genes, as well as contacted and transcribed regulatory elements, reach maximal expression at the same timepoints. Anchor sites of circadian gene promoter loops are enriched in DNA binding sites for liver nuclear receptors and other transcription factors, some exclusively present in either rhythmic or stable contacts. Finally, by comparing the interaction profiles between core clock and output circadian genes, we show that core clock interactomes are more dynamic compared to output circadian genes.ConclusionOur results identify chromatin conformation dynamics at different scales that parallel oscillatory gene expression and characterize the repertoire of regulatory elements that control circadian gene transcription through rhythmic or stable chromatin configurations.

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The global and promoter-centric 3D genome organization temporally resolved during a circadian cycle

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-05-04 | GSE155161 | GEO