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Deciphering Endoderm-derived Macrophage-like Metaphocytes in Zebrafish Gill and Intestine

ABSTRACT: Deciphering Endoderm-derived Macrophage-like Metaphocytes in Zebrafish Gill and Intestine

PROVIDER: PRJNA649247 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12340116_1.fastq.gz	Fastqsanger.gz
	SRR12340116_2.fastq.gz	Fastqsanger.gz
	SRR12340117_1.fastq.gz	Fastqsanger.gz
	SRR12340117_2.fastq.gz	Fastqsanger.gz
	SRR12340118_1.fastq.gz	Fastqsanger.gz

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Deciphering Endoderm-derived Macrophage-like Metaphocytes in Zebrafish Gill and Intestine

Project description:In order to have a comprehensive understanding of endoderm derived mpeg1+ cells in zebrafish gill and intestine, we isolated endoderm-derived GFP+ cells and remaining DsRedx+ cells (hematopoiesis-derived macrophages) from the gill (sox17_gill_GFP, sox17_gill_DsRedx) and intestine (sox17_intestine_GFP, sox17_intestine_DsRedx) of 4-OHT treated Tg(sox17:CreERT2;mpeg1:loxP-DsRedx-loxP-GFP) fish and performed RNA-seq. Both T-SNE analysis and feature gene comparison indicate that the endoderm-derived mpeg1+ cells in the gill and intestine are highly similar to the metaphocytes in the epidermis. We hence also refer to these endoderm-derived mpeg1+ cells as metaphocytes.

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