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Combinatorial action of temporally-segregated transcription factors

ABSTRACT: Combinatorial action of temporally-segregated transcription factors

PROVIDER: PRJNA649565 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12345938.fastq.gz	Fastqsanger.gz
	SRR12345939.fastq.gz	Fastqsanger.gz
	SRR12345940.fastq.gz	Fastqsanger.gz
	SRR12345941.fastq.gz	Fastqsanger.gz
	SRR12345942.fastq.gz	Fastqsanger.gz

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Combinatorial action of temporally-segregated transcription factors

Project description:Combinatorial action of transcription factors(TFs) with partially-overlapping expression is a widespreadstrategyto generate novel gene-expression patternsand thuscellular diversity. Known mechanisms underlying combinatorialactivityrequireco-expression of TFswithin the same cell. Here, we describe themechanismby which twoTFsthat are never co-expressed, generate a new, intersectional expression pattern in C. elegans embryos:lineage-specific priming of a gene by a transiently-expressedTFgeneratesa uniqueintersectionwith a second TF acting on the same gene four cell divisions later;the second TF is expressed in multiple cells butonly activatestranscription in those where priming occurred.Early induction of active transcription is necessary and sufficient to establish a competent state that is maintained by broadly-expressed regulators in absence of theinitial trigger. We further uncover new cells diversified through this mechanism. Our findings define anovel mechanism forcombinatorial TF activity with important implications for generation of cell-type diversity in vivo and in vitro.

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Combinatorial Action of Temporally Segregated Transcription Factors.

Project description:Combinatorial action of transcription factors (TFs) with partially overlapping expression is a widespread strategy to generate novel gene-expression patterns and, thus, cellular diversity. Known mechanisms underlying combinatorial activity require co-expression of TFs within the same cell. Here, we describe the mechanism by which two TFs that are never co-expressed generate a new, intersectional expression pattern in C. elegans embryos: lineage-specific priming of a gene by a transiently expressed TF generates a unique intersection with a second TF acting on the same gene four cell divisions later; the second TF is expressed in multiple cells but only activates transcription in those where priming occurred. Early induction of active transcription is necessary and sufficient to establish a competent state, maintained by broadly expressed regulators in the absence of the initial trigger. We uncover additional cells diversified through this mechanism. Our findings define a mechanism for combinatorial TF activity with important implications for generation of cell-type diversity.

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Temporally defined neocortical translation and polysome assembly is determined by the RNA-binding protein, Hu antigen R

Project description:Precise spatiotemporal control of mRNA translation machinery is essential to proper development of highly complex systems like the neocortex. Here, we show that an RNA-binding protein, Hu antigen R (HuR), regulates both neocorticogenesis and specificity of neocortical translation machinery in a developmental stagedependent manner in mice. Neocortical absence of HuR alters the phosphorylation states of the initiation and elongation factors of the core translation machinery. In addition, HuR regulates the temporally specific positioning of functionally related mRNAs into the active translation sites, the polysomes. HuR also determines the specificity of neocortical polysomes by defining their combinatorial composition of ribosomal proteins and initiation and elongation factors. For some of the HuR-dependent proteins, the association with polysomes depends on the eIF2 alpha kinase 4 (eIF2ak4), which associated with HuR in prenatal developing neocortices. Finally, we found that deletion of HuR prior to embryonic day 10 (E10) disrupts both neocortical lamination and formation of the main neocortical commissure, the corpus callosum. Our study identifies a crucial role for HuR in neocortical development as a translational gatekeeper for functionally related mRNA subgroups and polysomal protein specificity. Cortex was dissected from mouse pups at embryonic day 13 (E13) or the day of birth (P0).

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Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment

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