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Genome-wide maps of JMJ28pro::JMJ28:3xFLAG in 14 days old arabidopsis

ABSTRACT: Genome-wide maps of JMJ28pro::JMJ28:3xFLAG in 14 days old arabidopsis

PROVIDER: PRJNA650516 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12375802_1.fastq.gz	Fastqsanger.gz
	SRR12375802_2.fastq.gz	Fastqsanger.gz
	SRR12375803_1.fastq.gz	Fastqsanger.gz
	SRR12375803_2.fastq.gz	Fastqsanger.gz
	SRR12375804_1.fastq.gz	Fastqsanger.gz

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Genome-wide maps of JMJ28pro::JMJ28:3xFLAG in 14 days old arabidopsis

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of JMJ28 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide JMJ28-binding maps of 14 days old arabidopsis. To reveal bound genes by JMJ28, chimeric protein JMJ28-3xFLAG was expressed under JMJ28 promoter in jmj28 (JMJ28pro:JMJ28:3xFLAG/ jmj28). ChIP was performed using anti-FLAG antibody (FLAG-M2, F1804; SIGMA), and ChIP DNA were analyzed by Illumina Novaseq 6000.

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