Project description:Transcription profiling by array of 10 days old Brassica rapa ssp. chinensis seedlings treated with 2mM methyl jasmonate by spraying and harvesting 48 hours past treatment

2015-01-24 | E-MTAB-2386 | biostudies-arrayexpress

Brassica rapa cultivar:rapa PI169061

Project description:Brassica rapa ssp. rapa PI169061 Resequencing genome sequencing

| PRJNA616866 | ENA

Brassica rapa cultivar:rapa PI207464

Project description:Brassica rapa ssp. rapa PI207464 Resequencing genome sequencing

| PRJNA616867 | ENA

Brassica rapa cultivar:rapa G31848

Project description:Brassica rapa ssp. rapa G31848 Resequencing genome sequencing

| PRJNA616865 | ENA

Brassica rapa cultivar:rapa PI662772

Project description:Brassica rapa ssp. rapa PI662772 Resequencing genome sequencing

| PRJNA616868 | ENA

Transcriptome analysis for leaf morphology in Brassica rapa

Project description:Among Brassica rapa, rapid cycling Brassica rapa and Brassica rapa inbred line Kenshin showed contrasting leaf morphology. To identify genes associated with leaf morphology, four distinct F2 progeny of RcBr X Kenshin cross and their parents were selected. Leaf samples were collected from 6 materials, isolated total RNA, and subjected to newly developved 135K microarray. Experiments were performed with three or two biologic

2018-05-15 | GSE114479 | GEO

Genome annotation improvement of Brassica rapa

Project description:The mapping and functional analysis of quantitative traits in Brassica rapa can be greatly improved with the availability of physically positioned, gene-based genetic markers and accurate genome annotation. In this study, deep transcriptome RNA sequencing (RNA-Seq) of Brassica rapa was undertaken with two objectives: SNP detection and improved transcriptome annotation. We performed SNP detection on two varieties that are parents of a mapping population to aid in development of a marker system for this population and subsequent development of high-resolution genetic map. An improved Brassica rapa transcriptome was constructed to detect novel transcripts and to improve the current genome annotation. Deep RNA-Seq of two Brassica rapa genotypesâR500 (var. trilocularis, Yellow Sarson) and IMB211 (a rapid cycling variety)âusing eight different tissues (root, internode, leaf, petiole, apical meristem, floral meristem, silique, and seedling) grown across three different environments (growth chamber, greenhouse and field) and under two different treatments (simulated sun and simulated shade) generated 2.3 billion high-quality Illumina reads. In this experiment, two pools were made, with one pool consisting of 66 samples collected from growth chamber and another pool consisting of 60 samples collected from greenhouse and field. Each pool was sequenced on eight lanes (total 16 lanes) of an Illumina Genome Analyzer (GAIIx) as 100-bp paired end reads.

2014-04-22 | E-MTAB-4276 | biostudies-arrayexpress

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