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Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

ABSTRACT: Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

PROVIDER: PRJNA655833 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12407372_1.fastq.gz	Fastqsanger.gz
	SRR12407372_2.fastq.gz	Fastqsanger.gz
	SRR12407373_1.fastq.gz	Fastqsanger.gz
	SRR12407373_2.fastq.gz	Fastqsanger.gz
	SRR12407374_1.fastq.gz	Fastqsanger.gz

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Methylome of galls formed by Meloidogyne javanica in Arabidopsis thaliana at 3 days post infection

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Project description:Root-knot nematodes (RKNs) induce inside the vascular cylinder the giant cells (GCs) imbibed into a gall. Gene-repression in early developing GCs could be facilitated by small RNAs (sRNA) as miRNAs. 24nt-sRNAs, rasiRNAs and 21-22nt-sRNAs can also mediate epigenetic mechanisms. Three sRNA libraries from 3dpi galls and three from uninfected root segments were sequenced following Illumina-Solexa technology. Three sRNA libraries from 3dpi galls and three from uninfected root segments were sequenced

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Transcription profiling of Arabidopsis thaliana ematode induced early giant cells and normalvascular root cells.

Project description:Giant cells (GCs)formed by meloidogyne javanica 3 days post infection were isolated by microaspiration from the Arabidopsis Col0 galls. Three independent biological replicates were used for hybridisation. The controls for comparisons were vascular cylinder tissues from uninfected root segments at equivalent positions to the galls in the infected roots. RNA extracted directly from either the controls or the GCs were amplified two times with a RNA polimerase linear amplification based approach

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