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Transcriptome-wide m6A methylome of intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol

ABSTRACT: Transcriptome-wide m6A methylome of intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol

PROVIDER: PRJNA656710 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12433265_1.fastq.gz	Fastqsanger.gz
	SRR12433265_2.fastq.gz	Fastqsanger.gz
	SRR12433266_1.fastq.gz	Fastqsanger.gz
	SRR12433266_2.fastq.gz	Fastqsanger.gz
	SRR12433267_1.fastq.gz	Fastqsanger.gz

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Transcriptome-wide m6A methylome of intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol

Project description:Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. N6-methyladenosine (m6A) is an important epitranscriptomic marker with high abundance in eukaryotic mammals mRNAs. However, the role of the m6A methylomes in DON damage is still poorly understood. Here, we investigated the m6A transcriptome-wide profile in intestinal porcine epithelial cells (IPEC-J2) with and without 1000 ng/mL DON treatment via m6A sequencing and RNA sequencing. In total, 5406 new m6A peaks appeared with the disappearance of 2615 peaks in DON-induced IPEC-J2. The unique m6A-modified genes in DON-induced IPEC-J2 were associated with TNF signaling pathway. We identified 733 differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated m6A peaks between DON-induced IPEC-J2 and normal IPEC-J2. Protein interaction network analysis and qPCR validation suggested that CSF2 probably acts as a promising new target for combating DON damage in IPEC-J2. Our first report of m6A transcriptome-wide map of IPEC-J2 cells presented here provides a starting roadmap for uncovering m6A functions that may affect DON infection.

2020-08-13 | GSE156078 | GEO

Gene Regulation of Intestinal Porcine Epithelial Cells IPEC‑J2 is Dependent on the Site of Deoxynivalenol Toxicological Action

Project description:Here we analysed different mechanisms of apical and basolateral deoxynivalenol (DON) toxicity reflected in the gene expression. We used the jejunum derived, polarized intestinal porcine epithelial cell line IPEC‑J2 as an in vitro cell culture model. Luminal and blood stream DON intoxication was mimicked by a DON application from the apical or basolateral compartment of ThinCert® membrane inserts for 72 h.

2012-12-31 | GSE33246 | GEO

Transcriptional effects of Deoxynivalenol on intestinal porcine epithelial cells (IPEC-J2)

Project description:Transcriptional effects of Deoxynivalenol on intestinal porcine epithelial cells (IPEC-J2)

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

Project description:To gain a more complete understanding of how porcine cathelicidin PR-39 influence the porcine intestinal epithelial cells, we profiled gene expression patterns in IPEC-J2 cell line in the presence or the absence of PR-39.

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Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2)

2014-06-28 | E-GEOD-58876 | biostudies-arrayexpress

Transcriptome Profile of Natural Product Deoxyshikonin Induced Gene Expression in IPEC-J2

Project description:To understand the immunomodulatory effects of deoxyshikonin (a natural derivative of well-known Chinese medicine shikonin) on porcine intestinal epithelium cells, the transcriptome analysis was performed to explore the transcriptional profile of porcine IPEC-J2 cells after deoxyshikonin treatment.

2021-11-30 | GSE174377 | GEO

Transcriptional effects of Deoxynivalenol on intestinal porcine epithelial cells (IPEC-J2) under low glucose condition

Project description:Transcriptional effects of Deoxynivalenol on intestinal porcine epithelial cells (IPEC-J2) under low glucose condition

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Gene Regulation of Intestinal Porcine Epithelial Cells IPECM-bM-^@M-^QJ2 is Dependent on the Site of Deoxynivalenol Toxicological Action

Project description:Here we analysed different mechanisms of apical and basolateral deoxynivalenol (DON) toxicity reflected in the gene expression. We used the jejunum derived, polarized intestinal porcine epithelial cell line IPECM-bM-^@M-^QJ2 as an in vitro cell culture model. Luminal and blood stream DON intoxication was mimicked by a DON application from the apical or basolateral compartment of ThinCertM-BM-. membrane inserts for 72M-BM- h. We compared the genome wide gene expression of untreated and DON-treated IPEC-J2 cells by the GeneChipM-BM-. Porcine Genome Array of Affymetrix.

2012-12-31 | E-GEOD-33246 | biostudies-arrayexpress

Global transcriptional response of porcine intestinal epithelial cell lines to Salmonella enterica serovar Typhimurium and Choleraesuis: IPEC-J2 infected with S. typhimurium

Project description:The GeneChip Porcine Genome Array was used to identify the transcriptional response upon either Salmonella typhimurium (ST) or Salmonella choleraesuis (SC) infection in two porcine epithelial cell lines (IPEC-J2, from jejunum and IPI-2I, from ileum) during 2 and 4 hours post infection. The objectives in this study were first, to identify the different response between the epithelial cell lines from different gut regions; second, to study how the Salmonella serotypes used could elicit a different host response; and third, to determine the effect of the time-points on the differentially gene expression.

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Global transcriptional response of porcine intestinal epithelial cell lines to Salmonella enterica serovar Typhimurium and Choleraesuis: IPEC-J2 infected with S. choleraesuis

2013-12-20 | GSE23057 | GEO