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ATAC-Seq Data of Adipose Stem Cells from Subcutaneouse Adipose Tissue of Normal-Weight Polycystic Ovary Syndrome Women Undergoing Adipogenesis in vitro

ABSTRACT: ATAC-Seq Data of Adipose Stem Cells from Subcutaneouse Adipose Tissue of Normal-Weight Polycystic Ovary Syndrome Women Undergoing Adipogenesis in vitro

PROVIDER: PRJNA656827 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12440561_1.fastq.gz	Fastqsanger.gz
	SRR12440561_2.fastq.gz	Fastqsanger.gz
	SRR12440562_1.fastq.gz	Fastqsanger.gz
	SRR12440562_2.fastq.gz	Fastqsanger.gz
	SRR12440563_1.fastq.gz	Fastqsanger.gz

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ATAC-Seq Data of Adipose Stem Cells from Subcutaneouse Adipose Tissue of Normal-Weight Polycystic Ovary Syndrome Women Undergoing Adipogenesis in vitro

Project description:In previous studies, multiple animal models (rhesus monkeys, sheep) have suggested a developmental programming effect of PCOS. The goal of this study was to examine whether developmental programming may accompany epigenetic changes by examining intrinsic epigenetic differences, specifically chromatin accessibility, in adipose stem cells (ASCs) from subcutaneous (SC) adipose tissue of PCOS women vs age- and BMI-matched control samples as they mature into adipocytes in vitro.

2020-08-13 | GSE156140 | GEO

Gene Expression Data of Adipose Stem Cells from Normal-Weight Polycystic Ovary Syndrome Women vs. Controls

Project description:In vitro studies of subcutaneous (SC) abdominal adipose stem cells (ASC) from women with polycystic ovary syndrome (PCOS) show altered ASC commitment to preadipocytes and differentiation to mature adipocytes related to hyperandrogenism. The goal of the study is to use microarrays to examine whether SC abdominal ASC gene expression are altered in normal-weight PCOS women and correlated with hyperandrogenemia and/or insulin resistance, which are prevalent clinical pathologies of PCOS.

2019-01-24 | GSE124226 | GEO

Transcriptome profiling identified dysregulated signaling related to adipogenesis, metabolism, and inflammation in polycystic ovary syndrome patients

Project description:Polycystic ovary syndrome (PCOS) is a female endocrine disorder characterized by hyperandrogenism, chronic anovulation, and polycystic ovaries. PCOS is often accompanied by symptoms such as insulin resistance, abdominal obesity, and chronic inflammation. Adipose tissue is a crucial endocrine organ involved in metabolic disorders. Metabolic issues and chronic inflammation in PCOS are linked to dysfunctional adipose tissue. Mesenchymal progenitor cells (MPCs) are the precursor cells of adipocytes and can regulate the immune system. In this study, we used induced pluripotent stem cells (iPSCs) from patients with PCOS to derive MPCs and compare the transcriptome profiles between PCOS and HC iPSC-derived MPCs. We also challenged iPSC-derived MPCs with testosterone to assess the impact of androgen on MPCs. We found that 1026 genes differed between PCOS and HC iPSC-derived MPCs. Gene set enrichment analysis showed adipogenesis and metabolic function were reduced, but the inflammatory response was raised in PCOS iPSC-derived MPCs. The critical signals for early adipogenesis, including TGFβ, BMP, WNT, and CEBPA, differed between PCOS and HC iPSC-derived MPCs. After adipogenic induction, mature adipocytes were lower in PCOS iPSC-derived MPCs than HC. Lipolysis, the process involved in fat metabolism, was lower in adipocytes derived from PCOS MPCs than in HC. The testosterone treatment results indicated that genes related to oxidative phosphorylation and fatty acid metabolism were upregulated in HC iPSC-derived MPCs but downregulated in PCOS iPSC-derived MPCs. Short-term androgen stimulation may benefit body functions in HC. The impact of testosterone varied among individuals with HC and PCOS, possibly because of a genetic tendency towards PCOS. This study explains important factors that help us understand PCOS.

2024-08-16 | GSE267287 | GEO

MicroRNA expression profiling of human adipose stromal cell (hASC) adipogenesis

Project description:Human abdominal adipose tissue was obtained with informed consent from a 33-year old Caucasian female (BMI = 32.96 Kg/m2) undergoing lipoaspiration. Adipose stromal cells (hASCs) were isolated and differentiated into adipocytes in vitro. Two technical replicates from 9 time points relative to induction of adipogenesis (day 0). Also, one sample from pre-adipocytes (day -2) grown without FGF.

2010-09-30 | E-GEOD-20699 | biostudies-arrayexpress

RNA-seq Data of Newly-Formed Adipocytes from Adipose Stem Cells of Normal-Weight Polycystic Ovary Syndrome Women vs. Controls

Project description:Normal-weight polycystic ovary syndrome (PCOS) women exhibit adipose tissue dysfunction in vivo accompanied by enhanced subcutaneous (SC) abdominal adipose stem cell (ASC) development to adipocytes with greater lipid accumulation per cell in vitro. The goal of this study was to determine whether this phenomenon is associated with abnormal adipogenic gene transcription using RNA-sequencing to examine differential transcription patterns in PCOS vs controls. We found enhanced activation of adipogenic and adipocyte function genes and decreased expression of genes within the Wnt signaling pathway. In this study we conclude that this altered expression of genes in PCOS cells may underlie their exaggerated lipid accumulation in vitro and could predispose to adipose dysfunction in vivo when caloric intake exceeds energy utilization.

2020-08-12 | GSE156067 | GEO

Expression profiling of human adipose stromal cell (hASC) adipogenesis

Project description:Human abdominal adipose tissue was obtained with informed consent from a 33-year old Caucasian female (BMI = 32.96 Kg/m2) undergoing lipoaspiration. Adipose stromal cells (hASCs) were isolated and differentiated into adipocytes in vitro. Two technical replicates from 9 time points realtive to induction of adipogenesis (day 0). Also, two technical replicated from pre-adipocytes (day -2) grown without FGF. Two individual replicates (day 1 and day 7) were removed due to failed hybridizations.

2010-09-30 | E-GEOD-20697 | biostudies-arrayexpress

Expression data from subcutaneous adipose tissue of lean polycystic ovary syndrome (PCOS) and normoandrogenic women

Project description:Lean polycystic ovary syndrome (PCOS) women have a greater proportion of android (abdominal) fat, increased numbers of small subcutaneous (SC) abdominal adipocytes and preferential intra-abdominal fat accumulation. This study examines whether abnormal gene expression of SC abdominal adipose stem cells (ASCs) from lean PCOS women underlies this altered abdominal adipose structure-function. In this dataset, we include the expression data obtained from PCOS and NL subcutaneous adipose tissue. Differential expression of at least 1.5-fold change (P<0.05) were obtained in 120 genes (48 upregulated, 72 downregulated) of SC abdominal ASCs from PCOS versus NL women

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Gene Expression Data of Adipose Stem Cells from Normal-Weight Polycystic Ovary Syndrome Women vs. Controls

Project description:Gene Expression Data of Adipose Stem Cells from Normal-Weight Polycystic Ovary Syndrome Women vs. Controls

| PRJNA516631 | ENA

Transcription profiling of human omental adipose tissue in women with polycystic ovary syndrome (PCOS)

Project description:CONTEXT: The polycystic ovary syndrome (PCOS) is frequently associated with visceral obesity, suggesting that omental adipose tissue might play an important role in the pathogenesis of the syndrome. OBJECTIVE: The objective was to study the expression profiles of omental fat biopsy samples obtained from morbidly obese women with or without PCOS at the time of bariatric surgery. DESIGN: This was a case-control study. SETTINGS: We conducted the study in an academic hospital. PATIENTS: Eight PCOS patients and seven nonhyperandrogenic women submitted to bariatric surgery because of morbid obesity. INTERVENTIONS: Biopsy samples of omental fat were obtained during bariatric surgery. MAIN OUTCOME MEASURE: The main outcome measure was high-density oligonucleotide arrays. RESULTS: After statistical analysis, we identified changes in the expression patterns of 63 genes between PCOS and control samples. Gene classification was assessed through data mining of Gene Ontology annotations and cluster analysis of dysregulated genes between both groups. These methods highlighted abnormal expression of genes encoding certain components of several biological pathways related to insulin signaling and Wnt signaling, oxidative stress, inflammation, immune function, and lipid metabolism, as well as other genes previously related to PCOS or to the metabolic syndrome. CONCLUSION: The differences in the gene expression profiles in visceral adipose tissue of PCOS patients compared with nonhyperandrogenic women involve multiple genes related to several biological pathways, suggesting that the involvement of abdominal obesity in the pathogenesis of PCOS is more ample than previously thought and is not restricted to the induction of insulin resistance.

2007-10-26 | E-GEOD-5090 | biostudies-arrayexpress

Expression profiling of human adipose stromal cell (hASC) adipogenesis

2010-09-30 | GSE20697 | GEO