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Stromal androgen and hedgehog signaling regulates stem cell niches in pubertal prostate development

ABSTRACT: Stromal androgen and hedgehog signaling regulates stem cell niches in pubertal prostate development

PROVIDER: PRJNA656954 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12446296.fastq.gz	Fastqsanger.gz
	SRR12446297.fastq.gz	Fastqsanger.gz

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Stromal androgen and hedgehog signaling regulates stem cell niches in pubertal prostate development

Project description:Androgen-signaling is essential for prostate development. However, how androgen action facilitates prostatic stem/progenitor initiated pubertal prostatic growth remains unclear. Here, we demonstrate that androgens regulate Shh-signaling to control the cellular niche in prostatic epithelial development. Selective deletion of androgen receptor (AR) in stromal Shh-responsive cells significantly impedes pubertal prostatic epithelial morphogenesis and growth. Dysregulation of developmental signaling networks revealed in both prostatic stromal and epithelial cells of AR-deficient mice. Specifically, deletion of AR yielded increased Gli1 expression in prostatic stromal cells, elevated Shh expression in adjacent epithelial cells and stark inhibition of prostate cell growth. Trajectory analysis revealed AR deletion induces abnormal differentiation patterns of prostatic epithelia. Recombination of prostatic epithelial cells with AR-deficient stromal Gli1-expressing cells fails to develop normal prostatic epithelia. These data demonstrate the decisive role of stromal AR in interacting with Shh-signaling in the cellular niche to control pubertal prostatic morphogenesis and growth.

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