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HIF1A signaling selectively supports proliferation of breast cancer in the brain [F1 vs parental]

ABSTRACT: HIF1A signaling selectively supports proliferation of breast cancer in the brain [F1 vs parental]

PROVIDER: PRJNA659856 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12532007_1.fastq.gz	Fastqsanger.gz
	SRR12532007_2.fastq.gz	Fastqsanger.gz
	SRR12532008_1.fastq.gz	Fastqsanger.gz
	SRR12532008_2.fastq.gz	Fastqsanger.gz
	SRR12532009_1.fastq.gz	Fastqsanger.gz

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HIF1A signaling selectively supports proliferation of breast cancer in the brain [F1 vs parental]

Project description:Blood-borne metastasis to the brain is a major complication of breast cancer, but cellular pathways that enable cancer cells to selectively grow in the brain microenvironment are poorly understood. We find that cultured circulating tumor cells (CTCs), derived from blood samples of women with advanced breast cancer and directly inoculated into the mouse frontal lobe, exhibit striking differences in proliferative potential in the brain. Derivative cell lines generated by serial intracranial injections acquire selectively increased proliferative competency in the brain, with reduced orthotopic tumor growth. Increased Hypoxia Inducible Factor 1A (HIF1A)-associated signaling correlates with enhanced proliferation in the brain, and shRNA-mediated suppression of HIF1A or drug inhibition of HIF-associated glycolytic pathways selectively impairs brain tumor growth while minimally impacting mammary tumor growth. In clinical specimens, brain metastases have elevated HIF1A protein expression, compared with matched primary breast tumors, and, in patients with brain metastases, hypoxic signaling in CTCs predicts decreased overall survival. The selective activation of hypoxic signaling by metastatic cancers in the brain may be an opportunity for therapeutic intervention.

2020-10-17 | GSE156940 | GEO

HIF1A signaling selectively supports proliferation of breast cancer in the brain [parental CTCs inVitro]

Project description:HIF1A signaling selectively supports proliferation of breast cancer in the brain [parental CTCs inVitro]

| PRJNA659859 | ENA

HIF1A signaling selectively supports proliferation of breast cancer in the brain [HIF1A KD]

Project description:HIF1A signaling selectively supports proliferation of breast cancer in the brain [HIF1A KD]

| PRJNA659858 | ENA

HIF1A signaling selectively supports proliferation of breast cancer in the brain [parental CTCs inVitro]

2020-10-17 | GSE156943 | GEO

HIF1A signaling selectively supports proliferation of breast cancer in the brain

Project description:HIF1A signaling selectively supports proliferation of breast cancer in the brain

| PRJNA659643 | ENA

HIF1A signaling selectively supports proliferation of breast cancer in the brain [HIF1A KD]

2020-10-17 | GSE156942 | GEO

HIF1A signaling selectively supports proliferation of breast cancer in the brain [brain vs mammary fat pad]

2020-10-17 | GSE156941 | GEO

HIF1A signaling selectively supports proliferation of breast cancer in the brain [brain vs mammary fat pad]

Project description:HIF1A signaling selectively supports proliferation of breast cancer in the brain [brain vs mammary fat pad]

| PRJNA659857 | ENA

HIF1A signaling selectively supports proliferation of breast cancer in the brain.

Project description:Blood-borne metastasis to the brain is a major complication of breast cancer, but cellular pathways that enable cancer cells to selectively grow in the brain microenvironment are poorly understood. We find that cultured circulating tumor cells (CTCs), derived from blood samples of women with advanced breast cancer and directly inoculated into the mouse frontal lobe, exhibit striking differences in proliferative potential in the brain. Derivative cell lines generated by serial intracranial injections acquire selectively increased proliferative competency in the brain, with reduced orthotopic tumor growth. Increased Hypoxia Inducible Factor 1A (HIF1A)-associated signaling correlates with enhanced proliferation in the brain, and shRNA-mediated suppression of HIF1A or drug inhibition of HIF-associated glycolytic pathways selectively impairs brain tumor growth while minimally impacting mammary tumor growth. In clinical specimens, brain metastases have elevated HIF1A protein expression, compared with matched primary breast tumors, and in patients with brain metastases, hypoxic signaling within CTCs predicts decreased overall survival. The selective activation of hypoxic signaling by metastatic breast cancer in the brain may have therapeutic implications.

| S-EPMC7725834 | biostudies-literature

F1 allele specific gene expression

Project description:By comparing mouse fibroblasts from two parental strains (Bl6 and Spretus) with fibroblasts from their first generation offspring (F1) we can detect allele specific expression of proteins. The Bl6 and Spretus lines are evolutionary distant and harbour many SNPs in their genomes which when synonomous we can detect on the protein level using mass spectrometry. By mixing SILAC labeled Bl6, Spretus and F1 offspring cell lines we can detect peptides shared between all three cell lines and also SNP peptides that are only expressed in the F1 cells and either Bl6 or Spretus cells. By comparing the abundance of the shared peptides and the SNP peptides we can quantify how much of a protein in the F1 cells that comes from the paternal or maternal allele. This data were then further compared to polysome profiling data. Azidohomoalanine labeling was used to enrich newly synthesized proteins from the three cell lines.

2015-08-18 | PXD002337 | Pride

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