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Study of bone marrow Nestin+ mesenchymal stromal cells in control and leukemic mice

ABSTRACT: Study of bone marrow Nestin+ mesenchymal stromal cells in control and leukemic mice

PROVIDER: PRJNA659931 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12539464_1.fastq.gz	Fastqsanger.gz
	SRR12539464_2.fastq.gz	Fastqsanger.gz
	SRR12539465_1.fastq.gz	Fastqsanger.gz
	SRR12539465_2.fastq.gz	Fastqsanger.gz
	SRR12539466_1.fastq.gz	Fastqsanger.gz

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Global transcriptome of bone marrow Nestin+ mesenchymal stromal cells in homoestatis and human xenografts at early and late time points

Project description:We analysed the transcriptional signature of bone marrow Nestin+ mesenchymal stromal cells extracted from the bone marrow of mice engrafted with human AML cell lines and compared it to the one of untransplanted mice

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RNA-seq transcriptome analysis of Nestin-GFP-peri and -GFP-retic bone marrow stromal cells

Project description:Cell cycle quiescence is a critical feature contributing to haematopoietic stem cell (HSC) maintenance. Although various candidate stromal cells have been identified as potential HSC niches, the spatial localization of quiescent HSC in the bone marrow (BM) remains unclear. Here, using a novel approach that combines whole-mount confocal immunofluorescence imaging technique and computational modelling to analyse significant tridimensional associations among vascular structures, stromal cells and HSCs, we show that quiescent HSCs associate specifically with small arterioles that are preferentially found in endosteal BM. These arterioles are ensheathed exclusively by rare Nestin-GFP-peri/NG2+ pericytes, distinct from sinusoid-associated Nestin-GFP-retic/LepR+ cells. The present RNA-seq study sought to obtain a comprehensive understanding of the differences between the two distinct HSC cellular niches. mRNA profiles of sorted Nestin-GFP-peri and -GFP-retic bone marrow stromal cells were generated from pooled mice in triplicate by Illumina HiSeq 2000 sequencing.

2013-10-10 | E-GEOD-48764 | biostudies-arrayexpress

RNA-seq transcriptome analysis of Nestin-GFP-peri and -GFP-retic bone marrow stromal cells

2013-10-10 | GSE48764 | GEO

Study of bone marrow Nestin+ mesenchymal stromal cells in control and leukemic mice

Project description:NRAS-G12D+ mice show reduced numbers of mesenchymal stromal cells together with increased apoptotic rates as compared to healthy controls.

2022-11-14 | GSE157038 | GEO

RNAseq for Nestin+ bone marrow stromal cells in normal and MLL-AF9 mice

Project description:Cumulative evidence points out to the importance of the bone microenvironment for leukaemic development. Our preliminary results show that bone marrow stem cells, identified by the expression of the intermediate filament protein nestin (Nestin+ cells), provide support and chemoresistance to leukaemic cells. The rationale for these experiments is that leukaemic/Nestin+ cells crosstalk might regulate Nestin+ cells genetic profile to become more supportive elements for leukaemia. Nestin expression level distinguish two different Nestin+ populations, which seem to possess distint characteristics. Therefore, we would like to separately study high-expressing (Nestin high) and low-expressing Nestin cells (Nestin low). From our in vitro results, Nestin+ cells seem to be providing detoxifying tools to leukaemic cells such as antioxidant aminoacids and enzymes. The aim of the project is to identify pathways and genes differentially regulated in Nestin+ high and/or low cells in a leukaemic background.

2020-11-24 | GSE140135 | GEO

RNAseq for Nestin+ bone marrow stromal cells in normal and MLL-AF9 mice

Project description:RNAseq for Nestin+ bone marrow stromal cells in normal and MLL-AF9 mice

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Expression profile in bone marrow Nestin-GFP cells

Project description:Expression profile analysis in steady-state bone marrow-derived GFP+ cells obtained from transgenic mice in which GFP expression is regulated under the nestin gene promoter

2010-07-15 | GSE21941 | GEO

Expression data from bone marrow stromal cells (CD45.1-Ter119-)

Project description:Recent studies suggest that bone marrow stromal cells are critical for forming a niche that maintains hematopoietic stem cells’ quiescence and adapt blood production to the organism’s needs. Alterations in the bone marrow niche are commonly observed in blood malignancies and directly contribute to the aberrant function of disease-initiating leukemic stem cells. We used microarrays to detail the global programme of stromal cells' gene expression during leukemic progression.

2021-09-21 | GSE120286 | GEO

Expression profile in bone marrow Nestin-GFP cells

Project description:Expression profile analysis in steady-state bone marrow-derived GFP+ cells obtained from transgenic mice in which GFP expression is regulated under the nestin gene promoter Three replicates

2010-07-15 | E-GEOD-21941 | biostudies-arrayexpress

Expression data in bone marrow mesenchymal stromal cells derived from acute myeloid leukemia patients

Project description:Acute myeloid leukemia (AML) is a heterogeneous clonal disorder of hematopoietic stem/progenitor cells characterized by excessive proliferation and subsequent accumulation of immature myeloid blasts, leading to impaired hematopoiesis in the bone marrow (BM). The progression of AML is closely linked to the crosstalk between leukemic cells and the BM microenvironment, in particular the mesenchymal stromal cells (MSCs). We compared the mRNA expression profile of BM-MSCs from newly diagnosed AML patients (n=3), relapsed AML patients (n=3) and healthy donor controls (n=3)

2021-11-26 | GSE122917 | GEO

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