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RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa

ABSTRACT: RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa

PROVIDER: PRJNA660414 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12553400.fastq.gz	Fastqsanger.gz
	SRR12553401.fastq.gz	Fastqsanger.gz
	SRR12553402.fastq.gz	Fastqsanger.gz
	SRR12553403.fastq.gz	Fastqsanger.gz
	SRR12553380.fastq.gz	Fastqsanger.gz

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RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa

Project description:To dissect the role of RCO-3 and COL-26 in glucose uptake and metabolism and to obtain a broad view of the mode of expression, we conducted high-throughput sequencing (RNA-Seq) of wild-type, Δcol-26, and Δrco-3 mycelia exposed to a gradient of glucose (0%, 0.05%, 0.5%, 2.0%) for 1 h. We found that the gene expression profiles of Δcol-26 and Δrco-3 mutants was dramatically different with that of WT strain. Transcriptional profiling analysis revealed that Δcol-26 and Δrco-3 mutants had similar transcriptional profiles, and both mutants had impaired response to a glucose gradient. The genes involved glycolytic pathway showed significantly decreased expression in both mutants. Our findings provide novel insight into the molecular basis of glucose uptake and signaling in filamentous fungi, which may aid in the rational design of fungal strains for industrial purposes.

2020-09-01 | GSE157186 | GEO

RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.

Project description:BackgroundLow- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinity glucose transport system consists of a low-affinity glucose transporter GLT-1 and two high-affinity glucose transporters HGT-1/HGT-2, which play diverse roles in glucose transport, carbon metabolism, and cellulase expression regulation. However, the regulation of this dual-transporter system in response to environmental glucose fluctuation is not yet clear.ResultsIn this study, we report that a regulation module consisting of a downstream transcription factor COL-26 and an upstream non-transporting glucose sensor RCO-3 regulates the dual-affinity glucose transport system in N. crassa. COL-26 directly binds to the promoter regions of glt-1, hgt-1, and hgt-2, whereas RCO-3 is an upstream factor of the module whose deletion mutant resembles the Δcol-26 mutant phenotypically. Transcriptional profiling analysis revealed that Δcol-26 and Δrco-3 mutants had similar transcriptional profiles, and both mutants had impaired response to a glucose gradient. We also showed that the AMP-activated protein kinase (AMPK) complex is involved in regulation of the glucose transporters. AMPK is required for repression of glt-1 expression in starvation conditions by inhibiting the activity of RCO-3.ConclusionsRCO-3 and COL-26 form an external-to-internal module that regulates the glucose dual-affinity transport system. Transcription factor COL-26 was identified as the key regulator. AMPK was also involved in the regulation of the dual-transporter system. Our findings provide novel insight into the molecular basis of glucose uptake and signaling in filamentous fungi, which may aid in the rational design of fungal strains for industrial purposes.

| S-EPMC7841889 | biostudies-literature

RCO

Project description:Identification of the putative RCO targets using RNA-seq

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Neurospora crassa strain:rco-1

Project description:Neurospora crassa rco-1 Gene Expression Profiling - rco-1_1 transcriptome

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Neurospora crassa strain:rco-1

Project description:Neurospora crassa rco-1 Gene Expression Profiling - rco-1_3 transcriptome

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Neurospora crassa strain:rco-1

Project description:Neurospora crassa rco-1 Gene Expression Profiling - rco-1_2 transcriptome

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Identification of candidate genes downstream of RCO using RNA-seq

Project description:Transcriptome comparison after RCO induction

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| PRJEB34644 | ENA

Cancer responses to targeted radionuclide therapy (TRT) and external beam radiotherapy (EBRT)

Project description:The vast majority of our knowledge regarding cancer radiobiology and the activation of radio-resistance mechanisms emerged from studies using external beam radiation therapy (EBRT). Yet, less is known about the cancer response to internal targeted radionuclide therapy (TRT). Our comparative proteomics and phosphoproteomics study analyzed response to TRT with lutetium-177 labeled minigastrin analogue [177Lu]Lu-PP-F11N (β- emitter) in comparison to EBRT (ɣ-rays) in CCKBR-positive cancer cells.

2022-09-07 | PXD036034 | Pride

Proliferation of Listeria monocytogenes L-form cells by formation of internal and external vesicles.

Project description:L-forms are cell wall-deficient bacteria that divide through unusual mechanisms, involving dynamic perturbations of the cellular shape and generation of vesicles, independently of the cell-division protein FtsZ. Here we describe FtsZ-independent mechanisms, involving internal and external vesicles, by which Listeria monocytogenes L-forms proliferate. Using micromanipulation of single cells and vesicles, we show that small vesicles are formed by invagination within larger intracellular vesicles, receive cytoplasmic content, and represent viable progeny. In addition, the L-forms can reproduce by pearling, that is, generation of extracellular vesicles that remain transiently linked to their mother cell via elastic membranous tubes. Using photobleaching and fluorescence recovery, we demonstrate cytoplasmic continuity and transfer through these membranous tubes. Our findings indicate that L-forms' polyploidy and extended interconnectivity through membranous tubes contribute to the generation of viable progeny independently of dedicated division machinery, and further support L-forms as models for studies of potential multiplication mechanisms of hypothetical primitive cells.

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