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LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [Repli-seq]

ABSTRACT: LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [Repli-seq]

PROVIDER: PRJNA661444 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12588570_1.fastq.gz	Fastqsanger.gz
	SRR12588570_2.fastq.gz	Fastqsanger.gz
	SRR12588571_1.fastq.gz	Fastqsanger.gz
	SRR12588571_2.fastq.gz	Fastqsanger.gz
	SRR12588572_1.fastq.gz	Fastqsanger.gz

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LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [Repli-seq]

Project description:The chromatin-based rules governing the selection and activation of replication origins remain to be elucidated. It is believed that DNA replication initiates from open chromatin domains, thus replication origins residing in regulatory elements that are located at open and active chromatin. However, we report here that lysine specific demethylase 1 (LSD1), which biochemically catalyzes H3K4me1/2 demethylation to favor chromatin condensation, interacts with the DNA replication machinery. We found that LSD1 level peaks in early S phase. We demonstrated that LSD1 promotes DNA replication by facilitating origin firing in euchromatic regions and through regulating replication timing. Indeed, euchromatic zones enriched in H3K4me2 are the preferred sites for pre-RC binding in early replication. Remarkably, LSD1 deficiency leads to a genome-wide switch from early to late in replication timing. We showed that LSD1-promoted DNA replication is mechanistically linked to the loading of TICRR (TopBP1-Interacting Checkpoint and Replication Regulator) onto the pre-RC and subsequent recruitment of the initiator Cdc45 during origin firing. Together, these results reveal an unexpected role for LSD1 in euchromatic origin firing and replication timing.

2022-03-07 | GSE157470 | GEO

LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [ChIP-seq]

2022-03-07 | GSE157469 | GEO

LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [RNA-seq]

2022-03-07 | GSE157468 | GEO

LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing

Project description:LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing

| PRJNA661437 | ENA

LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [ChIP-seq]

Project description:LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [ChIP-seq]

| PRJNA661442 | ENA

LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [RNA-seq]

Project description:LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing [RNA-seq]

| PRJNA661443 | ENA

LSD1 Facilitates Euchromatic Origin Firing and Regulates Replication Timing

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-03-07 | GSE157471 | GEO

genome-wide analysis of replication timing by repli-chip and repli-seq

Project description:genome-wide analysis of replication timing by repli-chip and repli-seq

| PRJNA376018 | ENA

Mouse ESC 46C replication analysis by repli-chip

Project description:Mouse ESC 46C replication timing has been assessed by repli-chip.

2017-07-28 | GSE95090 | GEO

Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast

Project description:How early- and late-firing origins are selected on eukaryotic chromosomes is largely unknown. Here we show that Mrc1, a conserved factor required for stabilization of stalled replication forks, selectively binds to the early-firing origins in a manner independent of Cdc45 and Hsk1 kinase in fission yeast. In mrc1∆ (and in swi1∆ to some extent), efficiency of firing is stimulated and its timing is advanced selectively at those origins that are normally bound by Mrc1. In contrast, the late or inefficient origins which are not bound by Mrc1 are not activated in mrc1∆. The enhanced firing and precocious Cdc45 loading at Mrc1-bound early-firing origins are not observed in a checkpoint mutant of mrc1, suggesting that non-checkpoint function is involved in maintaining the normal program of early-firing origins. We propose that pre-firing binding of Mrc1 is an important marker of early-firing origins which are precociously activated by the absence this protein. Mrc1 binding profiles at G1/S boundary or early S-phase in wild type vs hsk1-89 mutant.

2011-03-26 | GSE28182 | GEO

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