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Transcriptome profiling of spinal cord vascular fragments of healthy mice that were either treated with imatinib or PBS

ABSTRACT: Transcriptome profiling of spinal cord vascular fragments of healthy mice that were either treated with imatinib or PBS

PROVIDER: PRJNA662115 | ENA |

REPOSITORIES: ENA

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Transcriptome profiling of vascular fragments at the preclinical, progression and remission phase of EAE in imatinib and PBS treated mice

Project description:Disruption of blood-brain barrier (BBB) integrity is a hallmark of several neurological disorders. Here we show that the BBB is dynamically and differentially affected during the preclinical, progression and remission phase of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). We isolated vascular fragments, representing the BBB, from spinal cords of imatinib and PBS treated mice at the preclinical, progression and remission phase of MOG-EAE as well as from non-immunized, naive mice.

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Transcriptome profiling of spinal cord vascular fragments of healthy mice that were either treated with imatinib or PBS

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Transcriptome profiling of vascular fragments in mice intracerebroventricular (ICV) injected with platelet derived dermal growth factor CC (PDGF-CC)

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Imatinib Disrupts Lymphoma Angiogenesis by Targeting Vascular Pericytes

2013-04-22 | E-GEOD-30752 | biostudies-arrayexpress

Spinal cord injury

Project description:Spinal cord injury

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Danio spinal cord injury

Project description:Adult zebrafish have the ability to recover from spinal cord injury and exhibit re-growth of descending axons from the brainstem to the spinal cord. We performed gene expression analysis using microarray to find damage-induced genes after spinal cord injury, which shows that Sox11b mRNA is up-regulated at 11 days after injury. However, the functional relevance of Sox11b for regeneration is not known. Here, we report that the up-regulation of Sox11b mRNA after spinal cord injury is mainly localized in ependymal cells lining the central canal and in newly differentiating neuronal precursors or immature neurons. Using an in vivo morpholino-based gene knockout approach, we demonstrate that Sox11b is essential for locomotor recovery after spinal cord injury. In the injured spinal cord, expression of the neural stem cell associated gene, Nestin, and the proneural gene Ascl1a (Mash1a), which are involved in the self-renewal and cell fate specification of endogenous neural stem cells, respectively, is regulated by Sox11b. Our data indicate that Sox11b promotes neuronal determination of endogenous stem cells and regenerative neurogenesis after spinal cord injury in the adult zebrafish. Enhancing Sox11b expression to promote proliferation and neurogenic determination of endogenous neural stem cells after injury may be a promising strategy in restorative therapy after spinal cord injury in mammals. Spinal cord injury or control sham injury was performed on adult zebrafish. After 4, 12, or 264 hrs, a 5 mm segment of spinal cord was dissected and processed (as a pool from 5 animals) in three replicate groups for each time point and treatment.

2010-10-19 | E-GEOD-20460 | biostudies-arrayexpress

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