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Functional analysis of caspase cleavable proteoforms of the Drosophila GSK-3 gene shaggy.

ABSTRACT: Functional analysis of caspase cleavable proteoforms of the Drosophila GSK-3 gene shaggy.

PROVIDER: PRJNA662249 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12606885.fastq.gz	Fastqsanger.gz
	SRR12606886.fastq.gz	Fastqsanger.gz
	SRR12606887.fastq.gz	Fastqsanger.gz
	SRR12606888.fastq.gz	Fastqsanger.gz
	SRR12606889.fastq.gz	Fastqsanger.gz

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Functional analysis of caspase cleavable proteoforms of the Drosophila GSK-3 gene shaggy.

Project description:A comparison of the embryonic transcriptome of wild type embryos (sggisoD-DS-SFS ) with embryos harbouring mutated DEVD caspase recognition sites ( sggisoD-cI-DS-SFS ) in a major shaggy isoforms class to examine the role of caspase cleavage in sgg function. The unique N-terminal exon undegoing caspase cleavage is tagged with DsRED at its N-terminus and StrepII-Flag-StrepII upstream of caspase cleavage sites for the wild type and caspase insensitive variant versions.

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