Project description:Transcriptional profiling of cancer stem cells (ALDH-high cells) comparing non-cancer stem cells (ALDH-low cells), sorted out using ALDEFLUOR assay. Goal was to identity cancer stem cell-specific genes. Two-condition experiment, sphere-cultured cells vs. adherent-cultured cells: 1 ALDH-high replicate and 1 ALDH-low replicate.

2016-04-01 | E-GEOD-77149 | biostudies-arrayexpress

Expression data of 4T1 mammary cancer cells cultured in MSC-conditioned medium or co-cultured with MSCs

Project description:4T1 mammary cancer cells exihibit higher proliferation and metastatic ability when cultured in conditioned medium from mesenchymal stem cells . Also, co-implantation with MSCs, 4T1 tumors show higher tumor growth and metastasis. We used microarrays to detail the global programme of gene expression underlying the alteration of cell behaviour RNA was extrated from 4T1 cells cultured in MSC-CM or regular medium as well as 4T1 cells isolated from co-cultured with MSCs. Gene expression of these cell groups was acquired from Affymetrix microarray analysis using mouse 430 2.0 array chip.

2012-06-06 | E-GEOD-38251 | biostudies-arrayexpress

Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

Project description:Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

2024-01-23 | GSE216080 | GEO

Expression data of 4T1 mammary cancer cells cultured in MSC-conditioned medium or co-cultured with MSCs

2012-06-07 | GSE38251 | GEO

RNA-seq of human normal mammary gland cells FACS sorted into three populations: ALDH+, ALDH-CD44+CD24-, and ALDH-CD44-CD24+

Project description:The intent of this experiment was to determine the similarities and differences in expression signatures of normal breast cells that express markers associated with an epithelial-like (ALDH+) and mesenchymal-like (CD44+CD24-) stem cells in the normal human breast. Briefly, tissues were collected from women undergoing voluntary reduction mammoplasty. Tissues were dissociated using chemical and enzymatic methods to yield single cells. These cells were sorted by flow cytometry for aldehyde hydrogenase activity (ALDH+), CD44, and CD24, as well as viability, following depletion of hematopoeitc cells, endothelial cells, and fibroblasts.

2018-01-17 | E-MTAB-5585 | biostudies-arrayexpress

ALDH RNA seq

Project description:ALDH RNA seq

| PRJNA835897 | ENA

Expression data from ALDH− and ALDH+ shXIst0SUM159 cells treated with or without DOX

Project description:We used microarrays to identify differentially expressed miRNAs in ALDH− and ALDH+ cells treated with or without DOX

2023-08-02 | GSE222742 | GEO