Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves

ABSTRACT: Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves

PROVIDER: PRJNA662626 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12619660_1.fastq.gz	Fastqsanger.gz
	SRR12619660_2.fastq.gz	Fastqsanger.gz
	SRR12619661_1.fastq.gz	Fastqsanger.gz
	SRR12619661_2.fastq.gz	Fastqsanger.gz
	SRR12619662_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves [scRNA-seq]

Project description:Leaves are asymmetric, with differential functionalization of abaxial and adaxial tissues. The bundle sheath (BS) surrounding the vasculature of the C3 crop barley is dorsoventrally differentiated into three domains: adaxial structural, lateral S-type, and abaxial L-type. S-type cells seem to transfer assimilates towards the phloem. Here we used single-cell RNA sequencing to investigate BS differentiation in C4 maize. Abaxial BS (abBS) cells of rank-2 intermediate veins specifically expressed three SWEET sucrose uniporters (SWEET13a, b, and c) and UmamiT amino acid efflux transporters. SWEET13a, b, c were also identified in the phloem parenchyma (PP). Thus maize acquired a unique mechanism for phloem loading in which abBS cells provide the main pathway for apoplasmic sucrose transfer towards the phloem. This pathway predominates in veins responsible for phloem loading (rank-2 intermediate), while rank-1 intermediate and major veins export sucrose from the phloem parenchyma (PP) adjacent to the sieve element companion cell (SE/CC) complex, as in Arabidopsis. We surmise that abBS identity is subject to dorsoventral patterning and has components of PP identity. The observations provide first insights into the unique properties of abBS cells, cells previously considered to fulfill the same functions as other bundle sheath cells (BSCs), and a basis for understanding the C4 syndrome.

2021-03-02 | GSE157757 | GEO

Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves [bulk RNA-seq]

2021-03-02 | GSE157758 | GEO

Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves [scRNA-seq]

Project description:Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves [scRNA-seq]

| PRJNA662627 | ENA

Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves [bulk RNA-seq]

Project description:Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves [bulk RNA-seq]

| PRJNA662628 | ENA

C4 Photosynthesis: Gene expression in mesophyll and bundle sheath cells of bundle sheath defective2 mutant

Project description:Zea mays is a C4 plant that utilizes two distinct cell types, mesophyll (M) and bundle sheath (BS), to cooperatively fix carbon. Regulation of M and BS cell differentiation is poorly understood. Here, we explore the transcriptional networks of M and BS cells by microarray analysis. The maize mutant bundle sheath defective2 lacks the accumulation of Rubisco small and large subunits (Roth et al 1996; Brutnell et al 1999) and cannot perform the Calvin Cycle (Smith et al 1998). Therefore, this mutant provides an opportunity to study M and BS cell differentiation in a perturbed BS background, potentially revealing regulons important to cell identity. M and BS cells were independently isolated from mutant and wild-type siblings. Transcriptional profiling was then performed in a cell specific manner between mutant and wild-type.

2018-04-30 | GSE13758 | GEO

Maize leaf development, bundle sheath

Project description:Band9 maize B73 leaf, Bundle sheath strand section1(0-1.5cm) biological replica1 SDS-PAGE trypsin Orbitrap

2011-04-15 | PRD000190 | Pride

RNAseq profiling of C4 grass Sorghum bicolor bundle sheath and mesophyll cells

Project description:Triplicate replicates of bundle sheath and mesophyll cell RNAseq from the C4 grass Sorghum bicolor.

2016-06-06 | E-MTAB-4021 | biostudies-arrayexpress

Bundle sheath and mesophyll cell RNAseq profiling

Project description:Bundle sheath and mesophyll cell RNAseq profiling

| PRJEB11652 | ENA

Single cell sequencing reveals phloem loading occurs via the abaxial bundle sheath cells in maize leaves

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-03-02 | GSE157759 | GEO

Transcriptome of Ustilago maydis during tumour formation in bundle sheath and mesophyll cells of Zea mays L.

Project description:The biotrophic fungus Ustilago maydis causes smut disease on maize (Zea mays L.), which is characterized by immense plant tumours. To establish disease and reprogram organ primordia to tumours, U. maydis deploys effector proteins in an organ-specific manner. However, the cellular contribution to leaf tumours remains unknown. We investigated leaf tumour formation on the tissue- and cell type-specific level. Cytology and metabolite analysis were deployed to understand the cellular basis for tumourigenesis. Laser-capture microdissection was performed to gain a cell-type specific transcriptome of U. maydis during tumour formation. In-vivo visualization of plant DNA synthesis identified bundle sheath cells as the origin of hyperplasic tumour cells, while mesophyll cells become hypertrophic tumour cells. Cell type specific transcriptome profiling of U. maydis revealed tailored expression of fungal effector genes. Moreover, U. maydis See1 was identified the first cell type specific fungal effector, being required for induction of cell cycle reactivation in bundle sheath cells. Identification of distinct cellular mechanisms in two different leave cell types, and See1 as an effector for induction of proliferation of bundle-sheath cells, are major steps in understanding U. maydis-induced tumor formation. Moreover, the cell-type specific U. maydis transcriptome data is a valuable resource to the scientific community.

2018-02-13 | GSE105770 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data