Salmonella enterica
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ABSTRACT:
PROVIDER: PRJNA662867 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Salmonella enterica subsp. enterica colistin resistance Genome sequencing
| PRJNA578046 | ENA
Project description:Colistin resistant Salmonella isolated from French food-animals and their environments
| PRJNA296856 | ENA
Project description:Acinetobacter baumannii is often highly resistant to multiple antimicrobials, posing a risk of treatment failure. Colistin is often chosen as a “last resort” for treatment of the bacterial infection, but resistance is easily developed when the bacteria is exposed to the drug. Thus a comprehensive analysis of colistin-mediated changes in colistin-susceptible and colistin-resistant A. baumannii is needed. In this study, we used a colistin-susceptible A. baumannii clinical isolate and a colistin-resistant isogenic mutant. Whole genome sequencing revealed that the resistant isolate harbored a PmrBL208F mutation conferring colistin resistance, and all other single nucleotide alterations were located in intergenic regions. Using scanning electron microscopy, we observed that the colistin-resistant mutant had a shorter cell length than the parental isolate, and filamented cells were observed when both isolates were exposed to inhibitory concentration of colistin. When the isolates were treated with inhibitory concentrations of colistin, more than 80% of the genes were upregulated, including genes associated with antioxidative stress response pathways. This results helped a better understanding for the morphological difference between the colistin-susceptible and –resistant isolates and differed colistin-mediated responses in A. baumannii isolates by their susceptibility to this drug.
2025-05-02 | GSE267612 | GEO
Project description:E.coli and Salmonella colistin resistance
| PRJEB18587 | ENA
Project description:Colistin is a crucial last-line drug used for the treatment of life-threatening infections caused by multi-drug resistant strains of the Gram-negative bacteria, Acinetobacter baumannii. However, colistin resistant A. baumannii isolates can be isolated following failed colistin therapy. Resistance is most often mediated by the addition of phosphoethanolamine (pEtN) to lipid A by PmrC, following missense mutations in the pmrCAB operon encoding PmrC and the two-component signal transduction system PmrA/PmrB. We recovered an isogenic pair of A. baumannii isolates from a single patient before (6009-1) and after (6009-2) failed colistin treatment that displayed low/intermediate and high levels of colistin resistance, respectively. To understand how increased colistin-resistance arose, we genome sequenced each isolate which revealed that 6009-2 had an extra copy of the insertion sequence element ISAba125 within a gene encoding an H-NS-family transcriptional regulator. Consequently, transcriptomic analysis of the clinical isolates identified was performed and more than 150 genes as differentially expressed in the colistin-resistant, hns mutant, 6009-2. Importantly, the expression of eptA, encoding a second lipid A-specific pEtN transferase, but not pmrC, was significantly increased in the hns mutant. This is the first time an H-NS-family transcriptional regulator has been associated with a pEtN transferase and colistin resistance.
2018-02-19 | GSE107964 | GEO
Project description:Salmonella enterica causes serious global burden of morbidity and mortality and is a major cause of infant bacteremia in sub Saharan Africa. Diseases caused by Salmonella are treatable with antibiotics but successful antibiotic treatment has become difficult due to antimicrobial resistance. An effective vaccine together with public health effort may therefore be a better strategy to control these infections. Protective immunity against Salmonella depends primarily on T cell-mediated immune responses and therefore identifying relevant T cell antigens is necessary for Salmonella vaccine development. Our laboratory has used an immunoproteomics approach to identify Chlamydia T cell antigens that exhibited significant protection against Chlamydia infection in mice. In this study, we infected murine bone marrow derived dendritic cells from C57BL/6 mice with Salmonella enterica strain SL1344 followed by isolation of MHC class I and II- molecules and elution of bound peptides. The sequences of the peptides were then identified using tandem mass spectrometry. We identified 87 MHC class II and 23 MHC class I Salmonella derived peptides. Four of 12 peptides stimulated IFN-? production by CD4 T cells from the spleens of mice with persistent Salmonella infection. These antigens will be useful for Salmonella immunobiology research and are potential Salmonella vaccine candidates.
2016-12-14 | PXD004451 | Pride
Project description:This experiment set includes 64 arrays representing 26 serovars and strains of Salmonella spp. including many representatives of subspecies I, Arizona from subsp. IIIa, and S. bongori from subsp. V. The genomic DNA from all strains were labeled with Cy5 and hybridized against an equal amount (1.5 ug) of S. typhimurium SL1344 reference genomic DNA that was labeled with Cy3, all on an S. typhimurium SL1344 spotted DNA microarray. Most of the arrays are present in triplicate to account for variability in probe generation, hybridization, and slide quality. Several are represented in duplicate, and a few without any replicates. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set
2005-11-04 | GSE3564 | GEO
Project description:Here we investigated colistin effects on A. baumannii, employing a Mass spectrometry approach and comparing a colistin-susceptible multidrug-resistantclinical isolate to its colistin-dependent subpopulation obtained by subsequent passages in moderate colistin concentrations. Colistin dependence might represent a stepping-stone to resistance, but the mechanisms of colistin dependence are far from clear.
2021-01-12 | PXD020218 | Pride
Project description:HLB SPE extract from S. enterica LT2 grown in TSB at 37C for 24hr. Media blank is included in supplementary files.
2022-07-31 | MSV000090032 | MassIVE
Project description:Salmonella enterica subsp. enterica serovar Rissen Genome with antibiotic-multinresistance profile including mcr-1 mediated resistance to Colistin sequencing and assembly
| PRJNA591084 | ENA