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Next Generation Sequencing Facilitates Quantitative Analysis of Cystine-regulated Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Cystine-regulated Transcriptomes

PROVIDER: PRJNA663304 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12637000_1.fastq.gz	Fastqsanger.gz
	SRR12637000_2.fastq.gz	Fastqsanger.gz
	SRR12637001_1.fastq.gz	Fastqsanger.gz
	SRR12637001_2.fastq.gz	Fastqsanger.gz
	SRR12637002_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Cystine-regulated Transcriptomes

Project description:Purpose: To identify differentially expressed genes regulated by cystine. Methods: HCT116 or RKO cells were cultured for 48 hours in cystine-free or 25μM cystine-containing conditional media, and three independent samples were collected by Trizol reagent and subjected to mRNA-sequencing by the LC Bio (Zhejiang, China) and data analysis Results: we identified cystine upregulated 145 genes and downregulated 169 genes in both HCT116 and RKO cells. GO enrichment analysis revealed that cystine regulated biological functions of G1/S transition of mitotic cell cycle and regulation of DNA replication biosynthetic process.In addition, KEGG enrichment analysis showed that mammalian target of rapamycin (mTOR) signaling pathway, a well-known nutrient sensor, was regulated by cystine. Furthermore,we noticed that SESN2 was one of the most significantly downregulated genes by cystine in both cell lines, and confirmed that cystine activates mTORC1 via the GCN2-ATF4-SESN2 axis in colon cancer cells. Conclusions: Our study established that cystine activates mTORC1 through GCN2-ATF4-SESN2 axis.

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Next Generation Sequencing Facilitates Quantitative Analysis of ISL1 Regulated Transcriptomes

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Next-Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in PB- and PB-TERRA-transfected 46C Mouse Embryonic Stem Cells

Project description:Next-generation sequencing facilitates quantitative analysis of transcriptomes in 46C mouse embryonic stem cells overexpressing PB-TERRA or PB empty vector at gene expression level. Results provide important information of the response of up-regulating TERRA, such as specific mechano-responsive genes, up- or down-regulated genes that were mainly enriched in signaling pathways regulating pluripotency of stem cells.

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Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in PB- and PB-Gbx2-transfected 46C mouse embryonic stem cells

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in 46C mouse embryonic stem cells overexpressing PB-Gbx2 or PB empty vector at gene expression level. Results provide important information of the response of up-regulating Gbx2, such as specific mechano-responsive genes, up- or down-regulated genes that were mainly enriched in signaling pathways regulating pluripotency of stem cells

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Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in PB- and PB-ID2-transfected 46C mouse embryonic stem cells

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