Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multiple mosquito species

ABSTRACT: Targeted amplicon sequencing of pathogens from mosquitoes

PROVIDER: PRJNA663912 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12796200_1.fastq.gz	Fastqsanger.gz
	SRR12796200_2.fastq.gz	Fastqsanger.gz
	SRR12796300_1.fastq.gz	Fastqsanger.gz
	SRR12796300_2.fastq.gz	Fastqsanger.gz
	SRR12796400_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 2358

Similar Datasets

Aedes aegypti strain:LVP_AGWG (yellow fever mosquito)

Project description:Aedes aegypti mosquitoes infect hundreds of millions of people each year with dangerous viral pathogens including dengue, yellow fever, Zika, and chikungunya. Progress in understanding the biology of this insect, and developing tools to fight it, depends on the availablity of a high-quality genome assembly. Here we use DNA proximity ligaton (Hi-C) and Pacific Biosciences long reads to create AaegL5 - a highly contiguous A. aegypti reference.

2018-08-23 | GSE113256 | GEO

mosquito metagenome

Project description:Anopheles stephensi and transgenic mosquitoes 16S profiling Targeted locus Metagenome

| PRJNA397763 | ENA

mosquito metagenome

Project description:Burkina Faso Anopheles 16s Targeted amplicon

| PRJNA265916 | ENA

Mosquito

Project description:Mosquitoes gut fungal microbiome Raw sequence reads

| PRJNA1062148 | ENA

Mosquito gene expression over time

Project description:A profile of gene expression of mosquitoes

2021-03-03 | E-MTAB-10110 | biostudies-arrayexpress

Production of Virus-Derived Ping-Pong-Dependent Piwi-like RNAs in the Mosquito Soma

Project description:The natural maintenance cycles of many mosquito-borne pathogens require establishment of persistent non-lethal infections in the invertebrate host. The mechanism by which this occurs is not well understood, but we have previously shown that an antiviral response directed by small interfering RNAs (siRNAs) is important in modulating the pathogenesis of alphavirus infections in the mosquito. However, we report here that infection of mosquitoes with an alphavirus also triggers the production of another class of virus-derived small RNAs that exhibit many similarities to ping-pong-dependent piwi-interacting RNAs (piRNAs). However, unlike ping-pong-dependent piRNAs that have been described previously from repetitive elements or piRNA clusters, our work suggests production in the soma and a role for dsRNA in biogenesis. We also present evidence that suggests virus-derived piwi-like RNAs are capable of modulating the pathogenesis of alphavirus infections in dicer-2 null mutant mosquito cell lines defective in viral siRNA production. Overall, our results suggest that a non-canonical piRNA pathway is present in the soma of vector mosquitoes and may be acting redundantly to the siRNA pathway to target alphavirus replication.

2012-01-06 | GSE32247 | GEO

Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

Project description:In this study we determine the effects of silencing adiponectin receptor in A. gambiae mosquitoes after taking Plasmodium gambiae infected blood meals. dsRNA (dsAdpR and dsGluc) microinjected A. gambiae mosquitoes were fed on P. berghei-infected Swiss Webster mice. Two days after a blood meal, the mosquitoes were collected for midgut dissection.

2023-11-09 | GSE198929 | GEO

Post-transcriptional microRNA modifications in the Dengue mosquito vector, Aedes aegypti

Project description:MicroRNAs (miRNA) have alternative forms known as isomiRs, which differ from each other by a few nucleotides. Next generation sequencing platforms facilitate identification of these isomiRs and recent discoveries regarding their functional importance have increased our understandings of the regulatory complexities of the microRNAome. Observed changes in the miRNA profiles in mosquitoes infected with flaviviruses have implicated small RNAs in the interactions between viruses and their vectors. Here we analysed the isomiR profiles of both uninfected and infected blood fed Aedes aegypti mosquitoes with a major human pathogen, Dengue virus at two time points post-infection. We found noticeable changes to the isomiR expression profile in response to infection and aging. Data analysis revealed a distinct bias towards isomiR production in the mature miRNA as opposed to the star strand. Furthermore, we noticed that only in 40% of Ae. aegypti miRNAs, the most abundant reads for each particular miRNA match the exact sequence reported in the miRbase. The isomiR expression variations between an Ae. aegypti embryonic cell line (Aag2) and whole mosquitoes demonstrated a tissue-specific pattern of isomiR production. Our results illustrated a bias for certain types of isomiRs for each miRNA. The findings presented in this study also provide evidence that isomiR production is not a random phenomenon and may be important in DENV colonisation of its vector.

2015-11-05 | GSE59516 | GEO

mosquito metagenome

Project description:Evolution of bacteria in anopheles mosquitoes

| PRJNA272632 | ENA

Production of Virus-Derived Ping-Pong-Dependent Piwi-like RNAs in the Mosquito Soma

2012-01-06 | E-GEOD-32247 | biostudies-arrayexpress