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Distinct roles of Bdh2 in regulating embryonic stem cell fate

ABSTRACT: Distinct roles of Bdh2 in regulating embryonic stem cell fate

PROVIDER: PRJNA664597 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12678525_1.fastq.gz	Fastqsanger.gz
	SRR12678525_2.fastq.gz	Fastqsanger.gz
	SRR12678526_1.fastq.gz	Fastqsanger.gz
	SRR12678526_2.fastq.gz	Fastqsanger.gz
	SRR12678527_1.fastq.gz	Fastqsanger.gz

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Distinct roles of Bdh2 in regulating embryonic stem cell fate

Project description:To further gain insights into the role of Bdh2 in cell fate decisions of mouse ESCs, we generated Bdh2 homozygous knockout mouse advanced pluripotent embryonic stem cell (ASC) and embryonic stem cell (ESC) lines by CRISPR/Cas9 genome editing technology. Bdh2 deficiency in ASCs/ESCs exhibited no effect on expression of core pluripotent transcription factors and alkaline phosphatase activity, suggesting dispensability of Bdh2 for self-renewal and pluripotency of embryonic stem cells. Notebly, Bdh2-knockout ASCs/ESCs exhibit higher expression of the endodermal somatic genes, Gata4, Gata6, Sox17,and also colocalizated with Oct4. Importantly, expression of key DNA methylation genes Dnmt3a, Dnmt3b and Dnmt1 decreased in Bdh2-knockout ASCs/ESCs. Overall, we provide insight on understanding of earliest cell fate decicion in ASCs/ESCs and a new avenue to create and preserve pioneer lineage precursors with pluripotency.

2020-09-21 | GSE158256 | GEO

Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition

Project description:Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition

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MLL1 and MLL fusion proteins play distinct roles in regulating MLLleukemic transcription program

Project description:MLL1 and MLL fusion proteins play distinct roles in regulating MLLleukemic transcription program

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Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition [RNA-seq]

Project description:Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition [RNA-seq]

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Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition [ChIP-seq]

Project description:Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition [ChIP-seq]

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Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition [scRNA-seq]

Project description:Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition [scRNA-seq]

| PRJNA971493 | ENA

A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate.

Project description:Glycogen synthase kinase 3 (GSK3) plays a central role in diverse cellular processes. GSK3 has two mammalian isozymes, GSK3α and GSK3β, whose functions remain ill-defined because of a lack of inhibitors that can distinguish between the two highly homologous isozymes. Here, we show that GSK3α and GSK3β can be selectively inhibited in mouse embryonic stem cells (ESCs) using a chemical-genetic approach. Selective inhibition of GSK3β is sufficient to maintain mouse ESC self-renewal, whereas GSK3α inhibition promotes mouse ESC differentiation toward neural lineages. Genome-wide transcriptional analysis reveals that GSK3α and GSK3β have distinct sets of downstream targets. Furthermore, selective inhibition of individual GSK3 isozymes yields distinct phenotypes from gene deletion, highlighting the power of the chemical-genetic approach in dissecting kinase catalytic functions from the protein's scaffolding functions. Our study opens new avenues for defining GSK3 isozyme-specific functions in various cellular processes.

| S-EPMC5851779 | biostudies-literature

Distinct roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5′TOP mRNAs

Project description:Distinct roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5′TOP mRNAs

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Fetal and trophoblast PI3K p110α have distinct roles in regulating resource supply to the growing fetus

Project description:Fetal and trophoblast PI3K p110α have distinct roles in regulating resource supply to the growing fetus

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Opposing roles for the lncRNA Haunt and its genomic locus in regulating HOXA gene activation during embryonic stem cell differentiation

Project description:Long noncoding RNAs (lncRNAs) have been implicated in controlling various aspects of embryonic stem cell (ESC) biology, although the functions of specific lncRNAs, and the molecular mechanisms through which they act, remain unclear. Here, we demonstrate discrete and opposing roles for the lncRNA transcript Haunt and its genomic locus in regulating the HOXA gene cluster during ESC differentiation. Reducing or enhancing Haunt expression, with minimal disruption of the Haunt locus, led to up- or down-regulation of HOXA genes, respectively. In contrast, increasingly large genomic deletions within the Haunt locus attenuated HOXA activation. The Haunt DNA locus contains potential enhancers of HOXA activation, whereas Haunt RNA acts to prevent aberrant HOXA expression. This work reveals a multi-faceted model of lncRNA-mediated transcriptional regulation of the HOXA cluster, with distinct roles for a lncRNA transcript and its genomic locus, while illustrating the power of rapid CRISPR/Cas9-based genome editing for assigning lncRNA functions. All RNA-seq(s) were designed to reveal the differentially expressed genes among different stages of ESCs differentiation, or differentially expressed genes between wild-type or Haunt or HOXA mutant cells during RA-induced differentiation. All ChIRP-Seq were used to reveal the DNA or RNA targets of Haunt before or after RA treatment.

2015-04-20 | E-GEOD-58514 | biostudies-arrayexpress

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