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ABSTRACT: Detection, replication and quantification of deformed wing virus-A, deformed wing virus-B, and black queen cell virus in the endemic stingless bee, Melipona colimana, from Jalisco, Mexico

PROVIDER: PRJNA664768 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12682738.fastq.gz	Fastqsanger.gz
	SRR12682739.fastq.gz	Fastqsanger.gz
	SRR12682740.fastq.gz	Fastqsanger.gz
	SRR12682741.fastq.gz	Fastqsanger.gz

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Project description:deformed wing virus

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Meta-analysis of honey bee neurogenomic response to deformed wing virus

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Effect of Deformed wing virus (DWV) and Varroa destructor (mite) on gene expression in honeybee (Apis mellifra) pupae

Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on global gene expression using microarray transcriptional profiling in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

2013-06-01 | E-MTAB-1285 | biostudies-arrayexpress

Meta-analysis of honey bee neurogenomic response to deformed wing virus (drone)

Project description:This dataset represents RNA-Seq data that was later found to have widespread contamination with Deformed Wing virus (DWV). It has been incorporated in a meta-analysis of DWV's impact on brain gene expression

2020-02-04 | GSE130702 | GEO

Meta-analysis of honey bee neurogenomic response to deformed wing virus (worker)

2020-02-04 | GSE130700 | GEO

Gene expression data for worker honey bees experimentally exposed to a dual infection with Deformed wing virus and Nosema ceranae.

Project description:Bees from 3 unrelated colonies were injected with 1ul PBS extract containing 10^9 genome equivalent of Deformed wIng virus (DWV) and/or fed 10Âµl sucrose solution containing 10^5 fresh Nosema ceranae spores. Control bees were injected and fed with an equivalent DWV- and Nosema-free extract respectively). Bees were kept in cages of 21 bees (7 from each colony), and each cage was replicated 5 times per each of the 4 treatments). Bess were kept in an incubator at 30Â°C/50%RH. At day 12 p.i., bees were flash frozen in liquid nitrogen, and stored at -80Â°C. Bee abdomen RNA was sent to Christina Grozinger lab (Penn State, USA). RNA was pooled for 3 abdomens per replicate for 5 replicates per treatment. Arrays were hybridized in a dye-swap loop design.

2017-03-08 | E-MTAB-5508 | biostudies-arrayexpress

Project description:RNA-Seq of deformed wing virus (DWV) infection on honey bee larvae

| PRJNA669279 | ENA

Meta-analysis of honey bee neurogenomic response to deformed wing virus (drone)

Project description:Meta-analysis of honey bee neurogenomic response to deformed wing virus (drone)

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Meta-analysis of honey bee neurogenomic response to deformed wing virus (worker)

Project description:Meta-analysis of honey bee neurogenomic response to deformed wing virus (worker)

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Varroa destructor LC-MSMS crossing developmental stages and sexes

Project description:Varroa destructor is one of the most prevalent and economically damaging honey bee pests worldwide, weakening colonies by simultaneously parasitizing and transmitting damaging viruses. Despite these impacts on honey bee health, surprisingly little is known about its fundamental molecular biology. Here we present a high-resolution V. destructor protein atlas crossing all major developmental stages (egg, protonymph, deutonymph and adult) for both male and female mites as a web-based interactive tool. In a proteogenomic effort, we identified 1,464 unique peptides corresponding to 419 proteins which were previously unannotated and we included these in all subsequent analyses. In order to use mass spectrometry-based peptide sequencing to augment the genome annotation of non-model species, we analyzed their amino acid and nucleotide composition as well as orthology to other species to suggest reasons why they may have been missed initially. Using label-free quantitative proteomics, we found that 1,433 proteins were differentially regulated across developmental stages, including proteins belonging to deformed wing virus and V. destructor virus. One other virus – the bee macula-like virus – was also detected, along with the protein generated by its short 3’ overlapping reading frame. In addition, we found that 101 proteins are sexually regulated and functional enrichment analysis suggests how they may contribute to sex-specific phenotypes and behaviour. Overall, this work provides a first of its kind interrogation of the patterns of gene expression that govern the Varroa life cycle and the tools we have developed will support further research on this threatening honey bee pest.

2017-09-06 | PXD006072 | Pride

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