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Effects of a Uty gene trap on the transcriptomic profile of mouse cardiac left ventricles in response to castration

ABSTRACT: Effects of a Uty gene trap on the transcriptomic profile of mouse cardiac left ventricles in response to castration

PROVIDER: PRJNA665031 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12692835.fastq.gz	Fastqsanger.gz
	SRR12692836.fastq.gz	Fastqsanger.gz
	SRR12692837.fastq.gz	Fastqsanger.gz
	SRR12692838.fastq.gz	Fastqsanger.gz
	SRR12692839.fastq.gz	Fastqsanger.gz

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Effects of a Uty gene trap on the transcriptomic profile of mouse cardiac left ventricles in response to castration

Project description:Previous results from the laboratory showed that the genetic make up of chromosome Y modulated the effects of castration on the transcriptome of mouse cardiac left ventricles. To test the effect of the chromosome Y Uty locus, the current study tests how castration affects the transcriptomes of cardiac left ventricles from adult male carrying a Uty gene trap (KO) in comparison to wild-type (WT)couterparts.

2021-09-19 | GSE158370 | GEO

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Left-right transcriptomic differences in adult male mouse heart ventricles

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Transcriptional profiling of ependymal cells lining the ventricles in the central nervous system of a Dcdc2a-TRAP (ES2575) mouse line

Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in ependymal cells lining the ventricles in the central nervous system of adult Dcdc2a-EGFP/Rpl10a (ES2575) mice

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Cardiac left ventricles of 12 week-old male C57BL/6J and C57BL/6J-chrY<A/J/NaJ> mice

Project description:We have reported previously that when chromosome Y (chrY) from the mouse strain C57BL/6J (abbreviated as B) was substituted for that of A/J mice (ChrY<A>), cardiomyocytes from the resulting 'chromosome substitution' C57BL/6J-chrY<A> strain (abbreviated as B.Y) were smaller than that of their C57BL/6J counterparts. In reverse, when chrY<A> from A/J mice was substituted for that of chrY<B>, cardiomyocytes from the resulting A/J-chrY<C57> strain were larger than in their A/J counterparts. We further used these strains (B and the consomic B.Y) to test whether the origin of chrY could also be linked to differences in the profile of gene expression in their cardiac left ventricles in adult mice where either sham surgery (intact animals) or castration has been performed at 3-4 weeks of age.. Samples from 4 different mice were used in each group. For hybridization, probes generated from samples were randomized on 2 different Illumina MouseRef v2.0 BeadChips.

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