Ontology highlight
ABSTRACT:
PROVIDER: PRJNA665162 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12763000_1.fastq.gz | Fastqsanger.gz | |||
| SRR12763000_2.fastq.gz | Fastqsanger.gz | |||
| SRR12763001_1.fastq.gz | Fastqsanger.gz | |||
| SRR12763001_2.fastq.gz | Fastqsanger.gz | |||
| SRR12763002_1.fastq.gz | Fastqsanger.gz |
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Project description:For phytophagous insects, the efficiency of utilization of hemicellulose and cellulose depends on the gut microbiota. Shifts in environmental and management conditions alter the presence and abundance of plant species which may induce adaptations in the diversity of gut microbiota. To test the adaptation of the microbiota to a shift from a natural diverse to a monocultural meadow with Dactylis glomerata the highly abundant grasshopper species, Chorthippus dorsatus, was taken from the wild and kept in captivity and were fed with Dactylis glomerata for five days. The feces were collected and analyzed by metaproteomics. After the diet shift from a diverse source to the single source, the microbiota composition stays relatively stable. The Bacilli as the group of highest abundance did not change on the functional level. In contrast, pronounced shifts of amino acid and carbohydrate metabolism in Clostridia and Proteobacteria were observed. Hence, the adaptation upon short-term change of food source in this grasshopper species is dominated by functional adaptations and not by shifts in the community structure of the microbiota. This suggests that the microbiota of grasshoppers is capable to cope also with the loss of diverse feeding plants at least for a shorter time period.
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Project description:Sexual selection on males is predicted to have widespread effects on genetic variation as a consequence of the pleiotropic allelic effects on sexual and non-sexual traits. We manipulated the opportunity for sexual selection on males during 27 generations of mutation accumulation in inbred lines of Drosophila serrata, and used a microarray platform to investigate the effect of sexual selection on the expression of 2689 genes. While gene expression signal was, on average, higher in the absence of sexual selection, this difference was small (0.1%). In contrast, sexual selection impacted substantially on the mutational variance in gene expression. Over all genes, mutational variance in gene expression was, on average, 42% higher when sexual selection operated than when it was absent. Our results indicate that sexual selection on males can generate widespread effects across the genome. An increase in mutational variance without a corresponding change in mean suggested that most expression traits were unlikely to be under direct sexual selection. Instead, the mutational variance in gene expression traits is consistent with divergence generated by widespread pleiotropic associations with traits affecting male mating success.
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