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INO80 promotes and safeguards bivalency [Mnase-seq]

ABSTRACT: INO80 promotes and safeguards bivalency [Mnase-seq]

PROVIDER: PRJNA665526 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12708204_1.fastq.gz	Fastqsanger.gz
	SRR12708204_2.fastq.gz	Fastqsanger.gz
	SRR12708205_1.fastq.gz	Fastqsanger.gz
	SRR12708205_2.fastq.gz	Fastqsanger.gz
	SRR12708206_1.fastq.gz	Fastqsanger.gz

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INO80 promotes and safeguards bivalency

Project description:INO80 promotes and safeguards bivalency

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INO80 promotes and safeguards bivalency [ATAC-seq]

Project description:INO80 promotes and safeguards bivalency [ATAC-seq]

| PRJNA665521 | ENA

INO80 promotes and safeguards bivalency [RNA-seq]

Project description:INO80 promotes and safeguards bivalency [RNA-seq]

| PRJNA665537 | ENA

INO80 promotes and safeguards bivalency [ChIP-seq]

Project description:INO80 promotes and safeguards bivalency [ChIP-seq]

| PRJNA665523 | ENA

INO80 promotes and safeguards bivalency [Mnase-seq]

Project description:INO80 is involved in many chromatin-dependent functions. However, its role in pluripotency has not been fully defined. We examined the impact of Ino80 deletion in the naïve and primed pluripotent stem cells. We found that Ino80 deletion had minimal effect on self-renewal and gene expression in the naïve state, but led to cellular differentiation and de-repression of developmental genes in the primed state. Mechanistically, INO80 co-occupied gene promoters that were bivalently marked by H3K4me3 and H3K27me3. Further, its occupancy was required for H3K27me3 installation and maintenance, as well as downstream gene repression. Finally, INO80 promoted H2A.Z occupancy at the bivalent domains, which in turn facilitated the polycomb repressive complex 2 (PRC2) recruitment. Together, our results identified the INO80-H2A.Z axis as an essential step for bivalent chromatin and poised gene expression and uncovered an epigenetic mechanism by which chromatin remodeling, histone variant, and histone modification coordinately control cell fate.

2021-07-04 | GSE158537 | GEO

INO80 promotes and safeguards bivalency [ChIP-seq]

2021-07-04 | GSE158533 | GEO

INO80 promotes and safeguards bivalency [RNA-seq]

2021-07-04 | GSE158544 | GEO

INO80 promotes and safeguards bivalency [ATAC-seq]

2021-07-04 | GSE158528 | GEO

INO80 promotes and safeguards bivalency

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-07-04 | GSE158545 | GEO

Proteomic analysis of histone modifications present on nucleosomes bound by INO80 complex

Project description:The INO80 complex is a chromatin remodeler that regulates DNA replication, repair, and transcription. Although the INO80 complex plays a crucial role in various chromatin-associated processes, the mechanism of its recruitment to specific genomic loci is not well understood. Here we used a native ChIP-MS approach to quantitatively profile modifications present on nucleosomes co-purified with INO80 from MNAse-digested HeLa chromatin.

2023-12-10 | PXD042210 | Pride

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