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Expression data from Staphylococcus aureus and Phosphate buffered saline (PBS) treated mouse wound bed

ABSTRACT: Expression data from Staphylococcus aureus and Phosphate buffered saline (PBS) treated mouse wound bed

PROVIDER: PRJNA665984 | ENA |

REPOSITORIES: ENA

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Expression data from Staphylococcus aureus and Phosphate buffered saline (PBS) treated mouse wound bed

Project description:Environmental factors that enhance regeneration are largely unknown. We hypothesized that skin bacteria modulate regeneration. Here, we assessed low, medium, and high levels of bacterial burden in Wound Induced Hair follicle Neogenesis (WIHN), a rare adult organogenesis model. WIHN levels and stem cell markers indeed correlated with bacterial counts, being lowest in germ free (GF), intermediate in conventional specific pathogen free (SPF), and highest even in mice infected with pathogenic Staphylococcus aureus. We identified IL-1β and keratinocyte-dependent IL-1R-MyD88 signaling as necessary and sufficient for bacteria to promote regeneration. Finally, in a small clinical trial, we found that a topical broad-spectrum antibiotic slowed skin wound healing. These results counter conventional notions that infection inhibits regeneration and the need for full sterility of small wounds.

2021-04-01 | GSE158614 | GEO

RNA-seq analysis of differentially expressed genes between stromal vascular fraction (SVF) from inguinal white adipose tissue (iWAT) of mice treated with medicinal herb extract (MHE) and Phosphate-buffered saline (PBS)

Project description:To assess the influences of a crude medicinal herb extract (MHE) on the immune composition and function in inguinal white adipose tissue (iWAT), we isolated iWAT stromal vascular fractions (SVFs) from control (PBS) and MHE-treated mice and performed RNA-seq analysis. iWAT SVF of mice treated with MHE was defined as the treatment group. Phosphate-buffered saline (PBS) treatment was used as the control group. Then RNA-Seq experiment was performed by OE Biotech Co., Ltd. (Shanghai, China) to analyze gene expression changes in iWAT SVF.

2024-10-09 | GSE279130 | GEO

Avoid Using Phosphate Buffered Saline (PBS) as an Electrolyte for Accurate OER Studies.

Project description: Not available

| S-EPMC11320652 | biostudies-literature

Analysis for expression of genes in human leukemic cell line Jurkat treated with Me6TREN or Phosphate Buffered Saline (PBS) control

Project description:mRNA from cells treated with or without small molecule Me6TREN for 24 hour was analyzed for differential gene expression. The hypothesis tested in the present study was that specific genes were up- or down-regulated via certain signaling pathways. Results provide important information to study the response of cellular functions triggered by Me6TREN. Total RNA obtained from human Jurkat cells subjected to 24 hours in vitro Me6TREN and PBS-treated control cells was amplified and hybridized to Illumina HT12 human whole-genome arrays.

2013-11-01 | E-GEOD-51974 | biostudies-arrayexpress

Vaseline and Neosporin treated human popliteal fossae (PF) skin wound bed

Project description:Vaseline and Neosporin treated human popliteal fossae (PF) skin wound bed

| PRJNA665985 | ENA

Analysis for expression of genes in human leukemic cell line Jurkat treated with Me6TREN or Phosphate Buffered Saline (PBS) control

2013-11-01 | GSE51974 | GEO

Preservation Method and Phosphate Buffered Saline Washing Affect the Acute Myeloid Leukemia Proteome.

Project description:Acute myeloid leukemia (AML) primary cells can be isolated from peripheral blood, suspended with media containing bovine serum and cryoprotectant, and stored in liquid nitrogen before being processed for proteomic analysis by mass spectrometry (MS). The presence of bovine serum and human blood proteins in AML samples can hamper the identifications of proteins, and thereby reduce the proteome coverage of the study. Herein, we have established the effect of phosphate buffered saline (PBS) washing on AML patient samples stored in media. Although PBS washes effectively removed serum and blood contaminants, the saline wash resulted in cell burst and remarkable protein material loss. We also compared different methods to preserve the AML proteome from THP-1 and Molm-13 cell lines before MS analysis: (1) stored in media containing bovine serum and dimethyl sulfoxide (DMSO); (2) stored as dried cell pellets; and (3) stored as cell lysates in 4% sodium dodecyl sulfate (SDS). MS analysis of differently preserved AML cell samples shows that preservation with DMSO produce a high number of fragile cells that will burst during freezing and thawing. Our studies encourage the use of alternative preservation methods for future MS analysis of the AML proteome.

| S-EPMC5796241 | biostudies-literature

Gene expression profiling of myofibroblasts in young and aged day 5 wound beds

Project description:Mesenchymal cells were isolated from day 5 dorsal skin wound beds of 7-weeks old and >24-months old using FACS. This experiment describes multiple unique subsets of wound bed myofibrolbasts capable of contributing to tissue repair.

2018-11-06 | GSE105790 | GEO

Influence of bovine serum albumin on corrosion behaviour of pure Zn in phosphate buffered saline.

Project description:Zinc (Zn) and its alloys have received increasing attention as new alternative biodegradable metals. However, consensus has not been reached on the corrosion behaviour of Zn. As cardiovascular artery stent material, Zn is supposed to contact with plasma that contains inorganic salts and organic components. Protein is one of the most important constitute in the plasma and could adsorb on the material surface. In this paper, bovine serum albumin (BSA) was used as a typical protein. Influences of BSA on pure Zn corrosion in phosphate buffered saline is investigated as a function of BSA concentrations and immersion durations by electrochemical techniques and surface analysis. Results showed that pure Zn corrosion was progressively accelerated with BSA concentrations (ranging from 0.05 to 5 g L-1) at 0.5 h. With time evolves, formation of phosphates as corrosion product was delayed by BSA adsorption, especially at concentration of 2 g L-1. Within 48 h, the corrosion of pure Zn was alleviated by BSA at concentration of 0.1 g L-1, whereas the corrosion was enhanced after 168 h. Addition of 2 g L-1 BSA has opposite influence on the pure Zn corrosion. Furthermore, schematic corrosion behaviour at protein/Zn interfaces was proposed. This work encourages us to think more about the influence of protein on the material corrosion and helps us to better understand the corrosion behaviour of pure Zn.

| S-EPMC8373726 | biostudies-literature

Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [scRNA-seq]

Project description:Tissue regeneration is a process that recapitulates the molecular and mechanical aspects of development and evolution. We use the wound-induced hair neogenesis (WIHN) model to investigate the mechanical and molecular responses of the laboratory (Mus) and African spiny (Acomys) mice. Laboratory and spiny mice showed an opposite trend of spatiotemporal morphogenetic field for WIHN during wound healing, and wound stiffness gradient across the whole wound bed predicated pattern of hair formation. Using bulk and single-cell RNA-seq analysis and K14-Cre-Twist1 transgenic mice, we identified the central role of the Twist1 pathway as the mediator of epidermal-dermal interaction and the emergence of periodic hair primordia. Lastly, we generated a Turing model with an underlying measure of stiffness to support a two-scale tissue mechanic model to explain the setup of a morphogenetic field from a wound bed (mm scale) or periodically arranged hair primordia from a morphogenetic field (μm scale). Delineating the common and distinct chemo-mechanical events during regenerative wound healing between laboratory and African spiny mice reveal its evo-devo advantages, which provide new perspectives for regenerative medicine.

2023-10-23 | GSE159827 | GEO

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