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A healthy human liver cell atlas reveals hepatic stellate cell heterogeneity

ABSTRACT: A healthy human liver cell atlas reveals hepatic stellate cell heterogeneity

PROVIDER: PRJNA666353 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12739443_1.fastq.gz	Fastqsanger.gz
	SRR12739443_2.fastq.gz	Fastqsanger.gz
	SRR12739444_1.fastq.gz	Fastqsanger.gz
	SRR12739444_2.fastq.gz	Fastqsanger.gz
	SRR12739445_1.fastq.gz	Fastqsanger.gz

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A healthy human liver cell atlas reveals hepatic stellate cell heterogeneity

Project description:We generated a high-resolution cellular atlas of the healthy human liver by profiling the transcriptome of more than 25,000 individual liver cells using droplet-based RNA-sequencing. Recently published datasets and in situ hybridization were integrated to confirm, validate and locate newly identified cell populations. We identified, annotated and characterized a total of 23 cell subpopulations that represent the degree of heterogeneity of parenchymal (i.e. hepatocytes and cholangiocytes) and non-parenchymal liver cells (i.e. endothelial cells, stellate cells, macrophages and lymphoid cells). We successfully classified human hepatocytes and liver sinusoidal endothelial cells along the porto-central axis and for the first time reveal the existence of functionally specialized pericentral GPC3+ and periportal HHIP+ DBH+ hepatic stellate cells in the healthy human liver. Our study provides a description of the different cell compartments that enter into the composition of a healthy human liver and currently constitutes the biggest single-cell RNA sequencing dataset available on human healthy hepatocytes and hepatic stellate cells. We identified subsets of hepatic stellate cells characterized by distinct localization and physiological functions.

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