Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Resistance to the herbicide 2,4-D in Sisymbrium orientale conferred by a deletion mutation in the degron tail of IAA2

ABSTRACT: Resistance to the herbicide 2,4-D in Sisymbrium orientale conferred by a deletion mutation in the degron tail of IAA2

PROVIDER: PRJNA667940 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12789810_1.fastq.gz	Fastqsanger.gz
	SRR12789810_2.fastq.gz	Fastqsanger.gz
	SRR12789811_1.fastq.gz	Fastqsanger.gz
	SRR12789811_2.fastq.gz	Fastqsanger.gz
	SRR12789812_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Resistance to the herbicide 2,4-D in Sisymbrium orientale conferred by a deletion mutation in the degron tail of IAA2

Project description:Resistance to the phenoxy synthetic auxin herbicide 2,4-D is conferred by a deletion mutation in the degron tail of IAA2. An RNA-Seq experiment was used to assemble a reference transcriptome for the weed species Sisymbrium orientale, to quantify gene expression, and to identify sequence variants in the alignments. A 27 bp deletion was identified in the IAA2 gene. Forward genetics supported the role of this deletion mutation in resistance. The IAA2 deletion allele was transformed into Arabidopsis thaliana to confirm that it conferred 2,4-D resistance.

2020-10-08 | GSE159202 | GEO

An in-frame deletion mutation in the degron tail of auxin coreceptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale.

Project description:The natural auxin indole-3-acetic acid (IAA) is a key regulator of many aspects of plant growth and development. Synthetic auxin herbicides such as 2,4-D mimic the effects of IAA by inducing strong auxinic-signaling responses in plants. To determine the mechanism of 2,4-D resistance in a Sisymbrium orientale (Indian hedge mustard) weed population, we performed a transcriptome analysis of 2,4-D-resistant (R) and -susceptible (S) genotypes that revealed an in-frame 27-nucleotide deletion removing nine amino acids in the degron tail (DT) of the auxin coreceptor Aux/IAA2 (SoIAA2). The deletion allele cosegregated with 2,4-D resistance in recombinant inbred lines. Further, this deletion was also detected in several 2,4-D-resistant field populations of this species. Arabidopsis transgenic lines expressing the SoIAA2 mutant allele were resistant to 2,4-D and dicamba. The IAA2-DT deletion reduced binding to TIR1 in vitro with both natural and synthetic auxins, causing reduced association and increased dissociation rates. This mechanism of synthetic auxin herbicide resistance assigns an in planta function to the DT region of this Aux/IAA coreceptor for its role in synthetic auxin binding kinetics and reveals a potential biotechnological approach to produce synthetic auxin-resistant crops using gene-editing.

| S-EPMC8892348 | biostudies-literature

Sisymbrium austriacum

Project description:Sisymbrium austriacum Variation

| PRJNA175834 | ENA

Sisymbrium irio

Project description:Sisymbrium irio Genome sequencing and assembly

| PRJNA202979 | ENA

Cichlasoma orientale

Project description:Cichlasoma orientale

| PRJNA996193 | ENA

Draconibacterium orientale

Project description:Draconibacterium orientale overview