Project description:Seperate White and Opaque Candida albicans populations were exposed to a temperature shift from 25 degrees to 37 degrees. Samples were then harvested at various time points to follow the effects of the shift on the two cell types, particularly the opaque cell type which switches to the white cell type after several hours at 37 degrees.

2011-05-08 | E-MEXP-2705 | biostudies-arrayexpress

Effect of histone H2Av depletion on temperature dependence of the Drosophila S2R+ cell transcriptome

Project description:The histone H2A variant H2A.Z has a crucial role in the regulation of temperature-dependent gene expression in the plant Arabidopsis thaliana. To evaluate whether the Drosophila homolog histone H2Av has a similar role in the control of the transcriptional response to temperature change, we depleted this histone variant by RNA interference in S2R+ cells and analyzed the effect on the transcriptome after temperature shifts to different temperatures (14, 25 and 30°C). For control, we performed analogous experiments using treatment with lacZ dsRNA instead of His2Av dsRNA.

2021-09-13 | GSE159171 | GEO

Temperature shift under anaerobic conditions

Project description:Temperature shift under anaerobic conditions

| PRJNA507430 | ENA

Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

2021-09-13 | GSE159170 | GEO

Prp4-1 temperature shift

Project description:Prp4-1 and wt strains were grown at 26°C to A600 of 1.0, then an equal volume of 48°C media was added to bring the temperature to 37°C. Both strains were allowed to grow at 37°C and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature. Keywords = splicing Keywords: time-course

2002-04-16 | GSE34 | GEO

Prp4-1 temperature shift

Project description:Prp4-1 and wt strains were grown at 26Â°C to A600 of 1.0, then an equal volume of 48Â°C media was added to bring the temperature to 37Â°C. Both strains were allowed to grow at 37Â°C and samples were taken at 0 (before shift), 5, 15, 30, 60, and 120 mins after shift to restrictive temperature. Keywords = splicing

2010-05-27 | E-GEOD-34 | biostudies-arrayexpress

Transcriptome changes in Drosophila HB10 cells in response to a temperature shift from 24 to 14°C

Project description:Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to distinct temperatures is predicted to involve complex regulation. Initially, to assess the effects of temperature change within the readily tolerated temperature range on the transcriptome, we have performed expression profiling with S2R+ cells, a hemocyte-like cell line derived from the ectothermic organism Drosophila melanogaster. To adress the cell-type specificity of the transcriptome change induced by a temperature downshift from the optimal temperature to a temperature at the lower end of the readily tolerated range, we have performed an anologous experiment with cultured Drosophila HB10 cells. These cells with similarities to adult muscle cell progenitors have recently been derived from Drosophila embryos with the method (Simcox et al. 2008. PLoS Genetics 4, e1000142) involving expression of activated ras using Act5C-GAL4 in combination with UASt-ras85D[V12].

2021-09-13 | GSE159173 | GEO

ChIP-seq analysis of M1BP in Drosophila S2R+ cells

Project description:The genomic distribution of a novel transcription factor called M1BP was determined in Drosophila S2R+ cells

2013-08-13 | GSE49842 | GEO