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Malaciobacter mytili LMG 24559

ABSTRACT: Arcobacter mytili LMG 24559 Genome sequencing project

PROVIDER: PRJNA66823 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7588213.fastq.gz	Fastqsanger.gz
	SRR7588214.fastq.gz	Fastqsanger.gz
	SRR7588215.fastq.gz	Fastqsanger.gz
	SRR7588215_1.fastq.gz	Fastqsanger.gz
	SRR7588215_2.fastq.gz	Fastqsanger.gz

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Project description:Background: Pantoea ananatis LMG 2665T synthesizes and utilizes acyl homoserine lactones (AHLs) for signaling. In this strain, short chain AHLs (C4 to C8) are produced by the EanI/R quorum sensing (QS) system that is involved in pathogenicity and biofilm formation. The complete set of genes regulated by the EanI/R system in P. ananatis LMG 2665T is still not fully known. In the present study, RNA-seq was used to analyze the transcriptome profiles controlled by the EanI/R system in this strain by comparing the wild type strain and its QS mutant 2665T ean∆I/R during lag and log stages. The RNA seq data was validated by RT qPCR. Results: The results showed that the EanI/R regulon in P. ananatis LMG 2665T comprised 144 genes, constituting 3.3% of the whole transcriptome under the experimental conditions in this study. The majority of genes regulated by the EanI/R system included genes for flagella assembly, bacterial chemotaxis, pyruvate metabolism, two component system, metabolic pathways, microbial metabolism and biosynthesis of secondary metabolites. Conclusions: This is the first study to identify the EanI/R QS regulon in P. ananatis LMG 2665T. Functional analysis of genes regulated the EanI/R system in LMG 2665T could help unveil genes that play a vital role in pathogenesis and survival strategies of this pathogen.

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