Project description:The spores of A. acidoterrestris cryopreserved at -80 ℃ were activated by inoculating in AAM medium and cultured at 45 ℃ to logarithmic phase [19]. The activated bacterial suspension was centrifuged and resuspended into pH 2.0, 2.5, 3.0, and 4.0 (Control) medium, which were correspondingly cultured in medium for 20 and 60 minutes, respectively. Scanning electron microscopy was used to monitor the bacterial activity and morphology different acid stress.Alicyclobacillus acidoterrestris (A. acidoterrestris) causes pasteurized acidic juice spoilage, resulting in a significant decline of juice quality, and causing economic losses. Exploration of A. acidoterrestris in response to acid stress could help control contamination caused by the bacteria. In this study, the mechanism of A. acidoterrestris in response to acid stress was studied by quantitative phosphoproteomics technique. Result showed that the phosphorylation of 40 proteins in A. acidoterrestris were closely related to the regulation of acid stress. The KEGG pathway enrichment analysis showed that the quorum sensing pathway which might involved in the perception of A. acidoterrestris was mainly enriched. we found that the up-regulation of Spo0A and YidC phosphorylation, which may resist acid stress by forming spores. The phosphorylation level of pyruvate kinase increased, which may improve bacterial acid stress resistance through formation of energy supply. The phosphorylation level of ABC transporter permease was significantly up-regulated, which may be part of the cell adaptation adjustment and contribute to the survival of A. acidoterrestris under acid stress. In summary, the molecular mechanism of acid stress regulation of A. acidoterrestris was proposed via quantitative phosphoproteomics, which provided a theoretical and experimental basis for further investigation acid resistance mechanism of A. acidoterrestris.

2025-01-18 | PXD058649 | Pride

Alicyclobacillus cycloheptanicus strain:SCH

Project description:Alicyclobacillus cycloheptanicus strain:SCH Genome sequencing

| PRJNA689874 | ENA

Alicyclobacillus acidocaldarius strain:LH1

Project description:Alicyclobacillus acidocaldarius strain:LH1 Genome sequencing

| PRJNA1128703 | ENA

Unveiling the complete genome sequence of Alicyclobacillus acidoterrestris DSM 3922T, a taint-producing strain.

Project description:Several species from the Alicyclobacillus genus have received much attention from the food and beverages industries. Their presence has been co-related with spoilage events of acidic food matrices, namely fruit juices and other fruit-based products, the majority attributed to Alicyclobacillus acidoterrestris. In this work, a combination of short and long reads enabled the assembly of the complete genome of A. acidoterrestris DSM 3922T, perfecting the draft genome already available (AURB00000000), and revealing the presence of one chromosome (4,222,202 bp; GC content 52.3%) as well as one plasmid (124,737 bp; GC content 46.6%). From the 4,288 genes identified, 4,004 sequences were attributed to coding sequences with proteins, with more than 80% being functionally annotated. This allowed the identification of metabolic pathways and networks and the interpretation of high-level functions with significant reliability. Furthermore, the additional genes of interest related to spore germination, off-flavor production, namely the vdc cluster, and CRISPR arrays, were identified. More importantly, this is the first complete and closed genome sequence for a taint-producing Alicyclobacillus species and thus represents a valuable reference for further comparative and functional genomic studies.

| S-EPMC9713406 | biostudies-literature

Surface Immunoproteomics Reveals Potential Biomarkers in Alicyclobacillus acidoterrestris.

Project description:Alicyclobacillus acidoterrestris is a major putrefying bacterium that can cause pecuniary losses in the global juice industry. Current detection approaches are time-consuming and exhibit reduced specificity and sensitivity. In this study, an immunoproteomic approach was utilized to identify specific biomarkers from A. acidoterrestris for the development of new detection methods. Cell surface-associated proteins were extracted and separated by 2-D (two-dimensional) gel electrophoresis. Immunogenic proteins were detected by Western blot analysis using antisera against A. acidoterrestris. Twenty-two protein spots exhibiting immunogenicity were excised and eighteen of the associated spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS). These proteins were observed to be involved in energy and carbohydrate metabolism, transmembrane transport, response to oxidative stress, polypeptide biosynthesis, and molecule binding activity. This is the first report detailing the identification of cell surface-associated antigens of A. acidoterrestris. The identified immunogenic proteins could serve as potential targets for the development of novel detection methods.

| S-EPMC6288362 | biostudies-literature

Alicyclobacillus acidocaldarius strain:CU92

Project description:Alicyclobacillus acidocaldarius genome sequencing

| PRJNA280536 | ENA