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Harvested primary hepatocytes exposed to either normoxia or hypoxia (1% O2)

ABSTRACT: Harvested primary hepatocytes exposed to either normoxia or hypoxia (1% O2)

PROVIDER: PRJNA668365 | ENA |

REPOSITORIES: ENA

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Harvested primary hepatocytes exposed to either normoxia or hypoxia (1% O2)

Project description:Hepatocytes endogenously express high levels of the enzyme tryptophan dehydrogenase (TDO2), in the current study we explore the effect of reduced oxygen on hepatocyte Tryptophan metabolism

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Gene expression in human umbilical cord vein and artery endothelial cells under physiological chronic normoxia (3% O2, PCN) and standard culture normoxia (21% O2, SCN)

Project description:Endothelial cells, and many other types of cells too, physiologically reside in low O2 environments (~ 2-13% O2 or 15-60 mmHg pO2; [PCN]) relevantly to atmospheric O2 (~ 21% or 160 mmHg pO2 at sea level) in vivo. Such PCN is critical for endothelial functions. The majority of our current knowledge regarding the cellular and signaling mechanisms governing endothelial functions, however, is built on endothelial models established under atmospheric O2 (~21% O2). Herein, we comapred the transcriptional profiles between HUVE and HUAE cells cultured and expanded under PCN (3% oxygen) and standard culture normoxia (21% O2).

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Gene expression in human umbilical cord vein and artery endothelial cells under physiological chronic normoxia (3% O2, PCN) and standard culture normoxia (21% O2, SCN)

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Transcriptomics revealed sex differences in human pulmonary microvascular endothelial cells in normoxia and hypoxia

Project description:To investigate sex differences at the transcriptome level in human pulmonary microvascular endothelial cells (HPMECs) from healthy male and female donors basally (in normoxia) and in hypoxic conditions. RNA-seq was performed on male (n=3) and female (n=4) HPMECs that were cultured in conditions of physiological shear stress (PMID: 36730645) in normoxia (21% O2) or in hypoxia (1% O2) for either 24 or 48 hours.

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Transcriptomic profiling of wild-type and Bmal1-/- myoblasts under normoxia or actue hypoxia conditions

Project description:Purpose: The goal of this study is to investigate the role of interplay between circadian clock and oxygen-sensing pathways in determining myogenic progenitor cell fate. Methods: Total RNAs were extracted from wild-type and Bmal1-/- myoblasts following exposure to normoxia (21% O2) or hypoxia (1% O2) for 6 hours, and subjected to RNA-sequencing. Results: There were significantly up-regulated (443 in normoxia versus 477 in hypoxia) and down-regulated (745 in normoxia versus 796 in hypoxia) genes in Bmal1-/- cells compared to wild-type, with a large degree of overlap between hypoxia and normoxia, although the fold change of differential gene expression was generally greater under hypoxia versus normoxia. Conclusion: Loss of Bmal1 in myoblasts leads to a premature differentiation-prone transcriptome, which was exaggerated following exposure to hypoxia.

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Comparison of hypoxia (4 % O2) cultured human embryonic stem cells (hESCs) to normoxia (21 % O2) cultured

Project description:We compared the transcriptome at gene expression level in hypoxic and normoxic conditions. The transcriptome of cells cultured at three different timepoints, 2h, 24 h and 7 days with three different cell lines in normoxia and hypoxia was compared 18 samples total, 3 replicates in each conditions, 2h, 24 h and 7day timepoints in hypoxia and normoxia

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Comparison of hypoxia (4 % O2) cultured human embryonic stem cells (hESCs) to normoxia (21 % O2) cultured

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Gene expression signature of WT siblings and hif1α mutants in normoxia and after hypoxia chamber incubation

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2023-04-10 | PXD041436 |

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