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Transcriptomes of maize seedlings in wild-type and Gβ knockout lines.

ABSTRACT: Transcriptomes of maize seedlings in wild-type and Gβ knockout lines.

PROVIDER: PRJNA669868 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12844636_1.fastq.gz	Fastqsanger.gz
	SRR12844636_2.fastq.gz	Fastqsanger.gz
	SRR12844637_1.fastq.gz	Fastqsanger.gz
	SRR12844637_2.fastq.gz	Fastqsanger.gz
	SRR12844638_1.fastq.gz	Fastqsanger.gz

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Transcriptomes of maize seedlings in wild-type and Gβ knockout lines.

Project description:Purpose: We aim to reveal maize transcriptomic changes in wild-type and Gβ knockout lines. Methods: RNA-seq was used to reveal transcriptome of maize biological replicates of wild-type and Gβ knockout lines. Results: Differnentically expressed transcritps were identified by the comparison of biological replicates of wild-type and Gβ knockout lines. Conclusions: We identified differentially expressed genes in Gβ knockout lines.

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Project description:Purpose: We aim to reveal maize transcriptomic changes with water and salinity treatment. Methods: RNA-seq were used to reveal transcriptome of maize biological replicates with water and salinity treatment. Results: Differentially expressed transcripts were identified by the comparison of biological replicates with water and salinity treatment. Conclusions: We identified differentially expressed genes in respone to salinity treatment in maize.

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Project description:Genome-wide and organ-specific landscapes of epigenetic modifications and their relationships to mRNA and smRNA transcriptomes in maize We report an integrated genome-wide analysis of DNA methylation, histone modifications, smRNAs and mRNA transcriptional activity, using maize as a model. We surveyed the epigenomes of the maize inbred line B73 in shoot and root tissue by Illumina/Solexa 1G parallel sequencing after digesting genomic DNA with a methylation-sensitive restriction enzyme and after conducting chromatin immunoprecipitations (ChIP) using antibodies that target specific histone modifications (H3K4me3, H3K9ac, H3K27me3, H3K36me3, respectively). Additionally, we profiled RNA pools (micro RNA (miRNA), siRNA and mRNA) using the same sequencing strategy. Keywords: Epigenetics, mRNA transcription and small RNAs H3K4me3, H3K9ac, H3K27me3, H3K36me3, DNA methylation and mRNA, small RNA profiled from shoots and roots of 14 day-old maize B73 seedlings

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