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Single cell RNA-seq analysis of endothelial cells (HUVEC) treated with IL1b and TGFb2 as a model of endothelial to mesenchymal transition (EndMT)

ABSTRACT: Single cell RNA-seq analysis of endothelial cells (HUVEC) treated with IL1b and TGFb2 as a model of endothelial to mesenchymal transition (EndMT)

PROVIDER: PRJNA670667 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12877250_1.fastq.gz	Fastqsanger.gz
	SRR12877250_2.fastq.gz	Fastqsanger.gz
	SRR12877251_1.fastq.gz	Fastqsanger.gz
	SRR12877251_2.fastq.gz	Fastqsanger.gz
	SRR12877252_1.fastq.gz	Fastqsanger.gz

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Single cell RNA-seq analysis of endothelial cells (HUVEC) treated with IL1b and TGFb2 as a model of endothelial to mesenchymal transition (EndMT)

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Project description:Endothelial-to-mesenchymal transition (EndMT) is a dynamic transformation process that has a functional impact upon pathological vascular remodelling. However, the molecular mechanisms that govern EndMT remain largely unknown. We modelled this process in vitro by exposing human primary endothelial cells to a combination of transforming growth factor-β2 (TGF- β2) and interleukin-1β (IL-1β). RNAseq was carried out to analyse the change of gene expression during the transition and define the transcriptional architecture of EndMT.

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Project description:Transcriptional profiling of Human Umbilical Vein Endothelial Cells (HUVEC) comparing untreated control cells with IL-1-treated cells with or without pre-treatment with DHA.

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