Project description:We aim to the investigate the role of tamoxifen in breast cancer progression. LCC2 and MCF-7 cells were used as the resistant and sensitive model.
Project description:To elucidate the molecular mechanism and signaling pathways that PinX1 is involved in, a Human LncRNA Array V2.0 (Arraystar, USA)-based genome wide screen of the alterations in lncRNA and mRNA expression profiles was performed in MCF-7 cells stably overexpressing PinX1. MCF-7 breast cancer cells were transfected with the pcDNA3.1-PinX1 and pcDNA3.1 empty vector and the microarray analysis were performed after the clonal selection of cells with a high expression level.
Project description:To elucidate the molecular mechanism and signaling pathways that PinX1 is involved in, a Human LncRNA Array V2.0 (Arraystar, USA)-based genome wide screen of the alterations in lncRNA and mRNA expression profiles was performed in MCF-7 cells stably overexpressing PinX1.
Project description:Acquired resistance to tamoxifen and fulvestrant is a challenging clinical problem in the treatment of hormone receptor-positive breast cancer patients. Non-coding RNAs (ncRNAs) could contribute to endocrine resistance but their specific roles in tamoxifen and fulvestrant resistance are not fully understood.