Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out

ABSTRACT: RNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out

PROVIDER: PRJNA671311 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12885553.fastq.gz	Fastqsanger.gz
	SRR12885554.fastq.gz	Fastqsanger.gz
	SRR12885555.fastq.gz	Fastqsanger.gz
	SRR12885556.fastq.gz	Fastqsanger.gz
	SRR12885557.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

RNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out

Project description:We recently identified variants in PRDM15 in affected individuals with proteinuric kidney disease. To determine the downstream pathways mediated by PRDM15 knock-down, PRDM15 knock-out immortalized podocyte cell lines were generated using CRISPR/Cas genome editing. Two different guide RNAs were utilized to generate two different cell lines, while a scramble guide RNA was utilized as control. We performed RNAseq and identified 151 differentially regulated genes, which coalesced upon pathways involved in renal development.

2021-02-01 | GSE159978 | GEO

polyA RNA-seq of SMCHD1 and LRIF1 knock-out immortalized myoblasts

Project description:polyA RNA-seq of SMCHD1 and LRIF1 knock-out immortalized myoblasts

| PRJNA769400 | ENA

Gene expression in a conditionally immortalized mouse podocyte cell line

Project description:The specialized glomerular epithelial cell (podocyte) of the kidney is a complex cell that is often damaged in glomerular diseases. Study of this cell type is facilitated by an in vitro system of propagation of conditionally immortalized podocytes. Here, genes that are differentially expressed in this in vitro model of podocyte differentiation are evaluated. Conditionally immortalized undifferentiated mouse podocytes were cultured under permissive conditions at 33*C. Podocytes that were differentiated at the non-permissive conditions at 37*C were used for comparison.

2010-08-28 | E-GEOD-23856 | biostudies-arrayexpress

The change of mRNA expression in murine immortalized podocyte after miR-26a silencing

Project description:The change of mRNA expression in murine immortalized podocyte were analyzed after miR-26a silencing. These results provide a basical information of molecular pathology in podocyte biology. Mouse podocytes immortalized by temperature sensitive SV40 were used. Podocyte cultures grown at 33 °C were trypsinized and then cultured with RPMI-1640 without antibiotics in 24-well plates at 60–70% confluence for 2 days. On day 3, an anti-miR negative control (40 pmol) or the miR-26a miRNA inhibitor (40 pmol) was transfected to podocytes. The cells were analyzed after culturing for 24 hour.

2014-10-01 | E-GEOD-51210 | biostudies-arrayexpress

The change of mRNA expression in murine immortalized podocyte after miR-26a silencing

2014-10-01 | GSE51210 | GEO

Glucocorticoid receptor ChIP-seq in an immortalized podocyte cell line

Project description:The identification of the glucocorticoid receptor cistrome in a conditionally immortalized human podocyte cell line developed by transfection with the temperature-sensitive SV40-T gene

2017-07-17 | E-MTAB-5238 | biostudies-arrayexpress

Gene expression in a conditionally immortalized mouse podocyte cell line

2010-08-28 | GSE23856 | GEO

polyA RNA-seq of SMCHD1 and LRIF1 knock-out immortalized myoblasts

Project description:polyA RNA-seq was performed on different SMCHD1 and LRIF1 knock-out myoblast clones to assess the role of SMCHD1 and LRIF1 in transcriptioanl regulation in somatic cells

2023-01-31 | GSE185511 | GEO

Glucocorticoid receptor ChIP-seq in an immortalized podocyte cell line

Project description:Glucocorticoid receptor ChIP-seq in an immortalized podocyte cell line

| PRJEB17855 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of control, SETD6 Knock-out, SETD3 knock-out and SETD6+SETD3 knock-out HeLa cells Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of control, SETD6 Knock-out, SETD3 knock-out and SETD6+SETD3 knock-out HeLa cells Transcriptomes

| PRJNA819059 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data