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BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (CUT&RUN)

ABSTRACT: BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (CUT&RUN)

PROVIDER: PRJNA671710 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12893153.fastq.gz	Fastqsanger.gz
	SRR12893154.fastq.gz	Fastqsanger.gz
	SRR12893155.fastq.gz	Fastqsanger.gz
	SRR12893156_1.fastq.gz	Fastqsanger.gz
	SRR12893156_2.fastq.gz	Fastqsanger.gz

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BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (ChIP-Seq)

Project description:Trimethylation of histone H3 lysine 27 (H3K27me3) is crucially involved for gene silencing, (epi)genome organization, cell-fate decision-making and development. To date, functional readout of H3K27me3 is viewed to be achieved mainly through a class of H3K27me3-recognizing chromodomains harbored within the chromobox (CBX) subunit of Polycomb repressive complex 1 (PRC1), which causes chromatin compaction and gene repression partly through histone H2A lysine 119 mono-ubiquitination. We here report that engagement of H3K27me3 by an evolutionarily conserved bromo adjacent homology (BAH) domain harbored within BAH domain-containing protein 1 (BAHD1) significantly contributes to optimal repression of the H3K27me3-demarcated genes in mammalian cells. BAHD1 assembles a NurD-like transcriptional corepressor complex. Abolishing the BAHD1BAH:H3K27me3 interaction by point mutagenesis interferes with BAHD1 binding to chromatin, leading to chromatin remodeling and derepression of Polycomb target genes. Mice carrying an H3K27me3-binding-defective mutation of Bahd1BAH causes marked embryonic lethality, indicating a requirement of this pathway for development. Altogether, this work demonstrates an H3K27me3-initiated signaling cascade that operates through a conserved BAH ‘reader’ module class in mammals.

2021-03-16 | GSE160060 | GEO

BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (CUT&RUN)

2021-03-16 | GSE160059 | GEO

BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (RNA-Seq)

2021-03-16 | GSE160058 | GEO

BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (ChIP-Seq)

Project description:BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (ChIP-Seq)

| PRJNA671709 | ENA

BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (RNA-Seq)

Project description:BAHD1 couples H3K27me3 to gene silencing via a conserved BAH reader module and associated corepressor complex in mammals (RNA-Seq)

| PRJNA671711 | ENA

A conserved BAH module within mammalian BAHD1 connects H3K27me3 to Polycomb gene silencing

Project description:Trimethylation of histone H3 lysine 27 (H3K27me3) is important for gene silencing, (epi)genome organization and organismal development. In a prevalent model, the functional “readout” of H3K27me3 in mammalian cells is achieved through the H3K27me3-recognizing chromodomain harbored within the chromobox (CBX) component of canonical Polycomb repressive complex 1 (cPRC1), which induces chromatin compaction and gene repression. Here, we report that binding of H3K27me3 by a Bromo Adjacent Homology (BAH) domain harbored within BAH domain-containing protein 1 (BAHD1) is required for overall BAHD1 targeting to chromatin and for optimal repression of the H3K27me3-demarcated genes in mammalian cells. Disruption of direct interaction between BAHD1-BAH and H3K27me3 by point mutagenesis leads to chromatin remodeling, notably, increased histone acetylation, at its Polycomb gene targets. Mice carrying an H3K27me3-interaction-defective mutation of Bahd1BAH causes marked embryonic lethality, showing a requirement of this pathway for normal development. Altogether, this work demonstrates an H3K27me3-initiated signaling cascade that operates through a conserved BAH “reader” module within BAHD1 in mammals.

2021-03-16 | GSE167079 | GEO

A conserved BAH module within mammalian BAHD1 connects H3K27me3 to Polycomb gene silencing

Project description:A conserved BAH module within mammalian BAHD1 connects H3K27me3 to Polycomb gene silencing

| PRJNA702926 | ENA

BAHCC1 couples H3K27me3 to gene silencing and tumorigenesis via a conserved BAH module [BAHCC1_KD_k27me3_293_ChIPseq]

Project description:Tri-methylation of histone H3 lysine 27 (H3K27me3) regulates transcriptional repression, cell-fate determination and differentiation. We report that a conserved Bromo-Adjacent Homology (BAH) module harbored within BAHCC1, a previously uncharacterized chromatin regulator, ‘recognizes’ H3K27me3 specifically and enforces silencing of H3K27me3-demarcated genes in mammalian cells. Biochemical, structural and ChIP-seq-based analyses demonstrate that direct ‘readout’ of H3K27me3 by BAHCC1 is achieved through a hydrophobic trimethyl-lysine-binding ‘cage’ formed by the BAH domain, mediating co-localization of BAHCC1 and H3K27me3-marked genes. BAHCC1 is significantly overexpressed in human acute leukemia and biochemically, BAHCC1 interacts with repressors SAP30BP and HDAC. In acute leukemia, depletion of BAHCC1, or disruption of the BAHCC1 BAH-mediated ‘readout’ of H3K27me3, causes de-repression of H3K27me3-targeted genes that are involved in tumor suppression and cell differentiation, leading to the suppressed tumor growth. In mice, introduction of a germ-line mutation at Bahcc1 to disrupt its H3K27me3 engagement causes postnatal lethality, supporting a role of this pathway in development. Collectively, this study unveils a novel H3K27me3-directed transduction pathway in mammal cells that relies on a conserved BAH ‘reader’, deregulation of which contributes to oncogenesis.

2020-09-28 | GSE151574 | GEO

BAHCC1 couples H3K27me3 to gene silencing and tumorigenesis via a conserved BAH module [Gw760_E2aPbx1_mouse_Rnaseq]

2020-09-28 | GSE151577 | GEO

BAHCC1 couples H3K27me3 to gene silencing and tumorigenesis via a conserved BAH module [GW760_K27me3_ChIPseq]

2020-09-28 | GSE151575 | GEO