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Siraitia grosvenorii strain:NongYuan B6

ABSTRACT: Siraitia grosvenorii strain:NongYuan B6 Organism overview

PROVIDER: PRJNA67181 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1054194_1.fastq.gz	Fastqsanger.gz
	SRR1054194_2.fastq.gz	Fastqsanger.gz
	SRR1054196.fastq.gz	Fastqsanger.gz
	SRR1055186_1.fastq.gz	Fastqsanger.gz
	SRR1055186_2.fastq.gz	Fastqsanger.gz

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Increased physical activity accompanies carbohydrate preference in a B6.CAST-17 sub-congenic mouse strain

Project description:A C57BL6/J (B6) x CAST/Ei (CAST) strain intercross was used to identify the first mammalian QTL for macronutrient-specific intake (carbohydrate and fat) and for total energy intake. A region on proximal Chromosome 17 revealed two significant QTL that co-localized for increased macronutrient intake-carbohydrate (Mnic1) and total kilocalorie intake (Kcal2), adjusted for body weight. An interval-specific congenic strain, B6.CAST-17, was then developed which verified the QTL. A new sub-congenic strain was developed which retained the linked traits. Important new findings emerged shows that this congenic interval confers an activity phenotype, i.e., mice carrying the differential segment have 20% higher spontaneous physical activity levels compared with the host B6 strain. We hypothesize that this Chromosome 17 QTL is either encoded by a single gene locus that determines both food intake and physical activity, or by two or more genes, each determining a sub-phenotype of energy balance. Microarray analysis of skeletal muscle and hypothalamus in congenic and wild type B6 mice was carried out to identify potential candidate genes for the activity and food intake behavior. Keywords: macronutrient-specific intake, sub-congenic strain

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